说明书：

EZB-miQP2-2x qPCR Mix for microRNA-中文操作说明书-2021.pdf

Introduction

The EZBioscience^® 2× qPCR mix for microRNA (ROX2 plus) uses a specially modified Taq DNA polymerase protected by a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based Real-time quantitative PCR (qPCR) for microRNA expression detection.

The mix is prepared at 2× reaction concentration, and is specially optimized for microRNA, which strikingly improves the microRNA amplification and reduces the non-specific amplification.

Meanwhile, the ROX2 passive reference dye (low ROX) is premixed in the mixture to make it an excellent ready to use qPCR reagent for microRNA expression detection. Besides, a universal 3’ qPCR primer is also supplied in this Kit.

It is recommended that the kit be used together with the EZBioscience^® EZ-press Cell to cDNA Kit  for microRNA, or microRNA Reverse Transcription Kit (Cat. No.: EZB-miRT1 or EZB-miRT2).

Components

*: Contain Hot-start DNA Polymerase, dNTPs, Mg2+, SYBR Green I dye, and premixed with and ROX2, to rectify the error of fluorescence signals between different wells.

Storage 

Store at -20℃.

Notice

Appropriate Real-time PCR instruments for this Kit are as follows:

For detailed information, please check the product manual.