说明书:
EZB-Probe-R2-EZ-Probe qPCR Master Mix(ROX2 plus)-中文操作说明书-2021.pdf
Introduction
The EZBioscience® EZ-Probe qPCR Master Mix uses a specially modified Taq DNA polymerase protected by Hot Start technique, and optimized qPCR buffer system to perform Probe based real-time quantitative PCR (qPCR). The mix is supplied at 2× concentration, and allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses.
Component
Component | EZB-Probe-R2 (500 Rxns) | EZB-Probe-R2-L (2,500 Rxns) |
2× EZ-Probe qPCR Master Mix*1,2 | 5 ml | 25 ml |
*1: Primers/probe is not supplied in this product.
*2: The ROX2 premixed is used to rectify the error of fluorescence signals between different wells.
Storage
All components should be stored at -20°C and protected from light. The quality of this product can be guaranteed for 18 months under recommended storage conditions.
Notice
Appropriate real-time PCR instruments for this kit are as follows:
Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™,Opticon®, Opticon 2, Chromo4™; Roche LightCyclerTM 96, Roche LightCyclerTM 480; Eppendorf Mastercycler® ep realplex, realplex 2s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Thermo Scientific PikoReal Cycler; Analytikjena qTOWER 3G; Cepheid SmartCycler®. |
ABI 7500, 7500 Fast, Quant-Studio 3, 5, 6, 7, 12K Flex, Dx, ViiA™7; Stratagene MX3000P™, MX3005P™, MX4000™. |
For detailed information, please check the product manual.