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上海宇劲生物孙经理 
·产品描述
EpiQuik常规蛋白DNA结合分析试剂盒（比色法）提供了一整套必需的试剂和方法，在体外研究蛋白质-DNA相互作用.整个分析过程只要3小时，就可以得到比别的试剂盒更加好的分析结果.本试剂盒适用于体外分析蛋白质-DNA相互作用，尤其是使用哺乳动物组织和细胞提取物监测转录因子的激活.
EpiQuik常规蛋白DNA结合分析试剂盒（比色法）专为检测细胞核提取物中转录因子的DNA结合活性而设计.在检测实验中，生物素标记的双链寡核苷酸含有能结合转录因子的DNA共有序列，这种寡核苷酸在结合分析缓冲液中与细胞核提取物进行孵育.有活性的转录因子能够结合到保守序列上，形成的寡核苷酸-蛋白复合物吸附到微孔表面.靶蛋白能被特性抗体识别，通过抗体显色剂反应系统的检测来测定比色度.该通用蛋白-DNA结合分析试剂盒（比色法）内主要产品组份包括：PD1 （10X Wash Buffer） PD2 （Assay Binding Buffer） PD3 （Antibody Dilution Buffer） PD4 （Developing Solution） PD5 （Stop Solution） 8-Well Assay Strips （with Frame） 用户指南手册。
·产品特点
1、操作十分快捷，3小时内可完成；
2、采取比色度法定量检测蛋白活性，不接触放射性材料，增加安全系数；
3、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析；
4、操作简便、结果可靠、统一的分析条件；
| Input Type: | Nuclear Extracts |
| Research Area: | Chromatin & Transcription |
| Target Application: | Activity Measurement |
| Vessel Format: | 96-Well Plate |
| 100% Guarantee: | 6 months |
The EpiQuik™ General Protein-DNA Binding Assay Kit (Colorimetric) is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vitro efficiently. The entire procedure can be completed within 3 hours and produces far more superior results than any competitor kits. The EpiQuik™ General Protein-DNA Binding Assay Kits are suitable for analyzing protein-DNA interactions in vitro, specifically for detecting transcription factor activation using mammalian tissue and cell extracts. The kit has the following advantages:
Principle & Procedure
The EpiQuik™ General Protein-DNA Binding Assay Kit (Colorimetric) is designed for measuring the transcription factor of DNA binding activity in nuclear extracts. In an assay with the use of this kit, a biotin-labeled double stranded oligonucleotide (oligo) containing DNA binding consensus sequence for the target transcription factor is incubated with nuclear extract in the binding assay buffer. Active form of transcription factor in the nuclear extract binds to its consensus sequence. Oligo-protein complex is then captured onto the assay microwell. The target protein can be recognized by a high affinity antibody and colorimetrically measured through detection antibody-color development reagent reaction system.
Product Citations
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Belli M et. al. (August 2019). FOXO1 negates the cooperative action of FOXL2C134W and SMAD3 in CYP19 expression in HGrC1 cells by sequestering SMAD3 Journal of the Endocrine Society.
Hekmat-Scafe DS et. al. (November 2016). Using yeast to determine the functional consequences of mutations in the human p53 tumor suppressor gene: An introductory course-based undergraduate research experience in molecular and cell biology. Biochem Mol Biol Educ.
Jang JY et. al. (February 2016). Targeting adenine nucleotide translocase-2 (ANT2) to overcome resistance to epidermal growth factor receptor tyrosine kinase inhibitor in non-small cell lung cancer. Mol Cancer Ther.
Koh J et. al. (October 2015). EML4-ALK enhances programmed cell death-ligand 1 expression in pulmonary adenocarcinoma via hypoxia-inducible factor (HIF)-1α and STAT3 OncoImmunology.
Gao Y The Univer et. al. (June 2014). Up-regulation of Hepatic VLDLR via PPARα Is Required for the Triglyceride-Lowering Effect of Fenofibrate. J Lipid Res.
Klengel T et. al. (January 2013). Allele-specific FKBP5 DNA demethylation mediates gene-childhood trauma interactions. Nat Neurosci. 16(1):33-41.
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