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上海宇劲生物孙经理 
·产品描述
EpiQuik 植物染色质免疫共沉淀（ChIP）试剂盒可以有效进行体外检测植物中蛋白质-DNA相互作用，操作方便.操作过程不到六小时就可以获得比其它试剂盒更好的结果.试剂盒获得的染色质可用来进行定性和定量PCR、southern 印迹以及DNA微阵列分析.
EpiQuik 植物染色质免疫共沉淀（ChIP）试剂盒提供了对植物细胞样品进行染色质免疫沉淀反应所需的所有试剂.并且，试剂盒中还有一种ChIP级二甲基组蛋白H3-K9抗体和一种阴性对照抗体（正常小鼠的IgG）.从细胞样品中提取出来的染色质进行适当的打断，然后加入到微孔中与其表面上吸附的抗体发生免疫反应.特异性结合到微孔上的DNA从抗体-捕获蛋白-DNA复合物上释放出来，翻转后，通过本公司专门设计的高速离心柱纯化.洗脱下来的DNA可用于随后的各种分析.该植物染色质免疫沉淀（ChIP）试剂盒 内主要产品组份包括：CP1 （Wash Buffer） CP2 （Antibody Buffer） CP3C （5X Lysis Buffer I） CP3D （Lysis Buffer II） CP3E （Lysis Buffer III） CP3F （Lysis Buffer IV） CP4 （ChIP Dilution Buffer） CP5 （DNA Release Buffer） CP6 （Reverse Buffer） CP7 （Binding Buffer） CP8 （Elution Buffer） Protease Inhibitor Cocktail （100X） Normal Mouse IgG （1 mg/ml） Anti-Dimethyl H3-K9 （1 mg/ml） Proteinase K （10 mg/ml） 8-Well Assay Strips （with frame） 8-Well Strip Caps F-Spin Column F-Collection Tube 用户指南手册。
·产品特点
1、市场上同类产品中最快捷的试剂盒，整个处理过程不到6小时；
2、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析；
3、附有DNA纯化柱子：大量减少不必要的重复劳动；
4、操作简便、结果可靠、统一的分析条件；
| Input Type: | Chromatin |
| Research Area: | Chromatin & Transcription |
| Target Application: | Immunoprecipitation |
| Vessel Format: | 96-Well Plate |
| 100% Guarantee: | 6 months |
The EpiQuik™ Plant ChIP Kit is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vivo efficiently. The entire procedure can be completed within 6 hours and produces far superior results than any competitor kits. The EpiQuik™ Plant ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, ChIP-Seq, and ChIP-on-chip. This kit has the following advantages:
See also a quick chart to compare ChIP kits.
Background Information
Protein-DNA interaction play a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. In plants, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of genes that effect homeostasis, development, and adaptation. Chromatin Immunoprecipitation (ChIP) offers an advantageous tool for identifying direct genomewide associations between specific regulatory proteins and their target genes. Unlike other methods such as EMASA, DNA microarrays, and report gene assays, which analyze direct interactions between protein and DNA in vitro, ChIP can detect that a specific protein binds to the specific sequences of a gene in living cells.
Principle & Procedure
This ChIP kit includeds all reagents required for carrying out a successful chromatin immunoprecipitation from plant cells. Particularly, this kit includes a ChIP-grade dimethyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured protein-DNA complex, reversed and purified through the specifically designed F-Spin Column. Eluted DNA can be used for various down-stream applications.
Starting Materials
Starting materials can include various plant tissue (flowers, leaves, young seedlings). In general, the input amount should be from 20 to 50 mg of plant tissue for each reaction.
Product Citations
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