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上海宇劲生物孙经理 
·产品描述
EpiQuik HAT活性/抑制性检测试剂盒提供了一整套必需的试剂和方法检测HAT的活性/抑制性，安全快捷，即时可用.本试剂盒可以检测很多物种（包括哺乳动物细胞/组织、植物以及细菌）HAT的活性/抑制性.
EpiQuik HAT活性/抑制性检测试剂盒专门为检测总HAT活性/抑制性而设计.在检测实验中，本公司特有组蛋白基质牢牢地结合在微孔表面.有活性的HAT与组蛋白基质结合，并使之乙酰化.乙酰化的基质被高结合活性的抗乙酰化组蛋白抗体识别.乙酰化组蛋白的量与HAT酶活成正比，可以通过类酶联免疫共沉淀的方法测定吸光度而得到.该HAT活性/抑制分析试剂盒内主要产品组份包括：HT1 （10X Wash Buffer） HT2 （HAT Substrate， 20 ug/ml） HT3 （HAT Standard， 20 ug/ml） HT4 （Acetyl Co-A， 30 mM） HT5 （HAT Assay Buffer） HT6 （Capture Antibody， 100 ug/ml） HT7 （Detection Antibody， 200 ug/ml） HT8 （Developing Solution） HT9 （Stop Solution） 8-Well Assay Strip （with Frame） 用户指南手册。
·产品特点
1、操作快速，3小时内可完成；
2、独创的比色度检测方法，不接触放射性物质，无需进行抽提和色谱分析；
3、通过定量检测被乙酰化的组蛋白底物来直接检测HAT活性和抑制性，因此避免了对HAT的假抑制性的影响；
4、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析；
5、操作简便、结果可靠、统一的分析条件；
| Input Type: | Nuclear Extracts, Purified Enzyme |
| Research Area: | Histone Acetylation |
| Target Application: | Activity Measurement |
| Vessel Format: | 96-Well Plate |
| 100% Guarantee: | 6 months |
The EpiQuik™ HAT Activity/Inhibition Assay Kit is a ready-to-use set of essential components needed to measure histone acetyltransferase activity/inhibition safely and quickly. It is suitable for measuring histone acetyltransferase activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria. The kit has the following advantages:
Principle & Procedure
The EpiQuik™ HAT Activity/Inhibition Assay Kit is designed for measuring total histone aceyltransferase activity or inhibition. In an assay with this kit, the unique histone substrate is stably captured on the strip wells. Active HATs bind to and acetylate histone substrate. The acetylated substrate can be recognized with a high affinity anti-acetylated histone antibody. The ratio or amount of the acetylated histone, which is directly proportional to HAT enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.
Product Citations
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