·产品描述

       EpiQuik通用乙酰化组蛋白H3-K9定量分析试剂盒（比色法）能够检测总体H3-K9的乙酰化状态，操作便捷.本试剂盒提供了进行检测实验所需的一整套试剂和方法，即时可用.EpiQuik通用乙酰化组蛋白H3-K9定量分析试剂盒（比色法）专门用来检测包括新鲜和冷冻组织、贴壁培养细胞以及细胞悬浮液在内各种哺乳动物细胞（人类、鼠类等）通用组蛋白H3-K9位置的乙酰化.

       EpiQuik通用乙酰化组蛋白H3-K9定量分析试剂盒（比色法）专门为检测通用组蛋白H3-K9的乙酰化而设计.检测实验中，而第9位赖氨酸被乙酰化的组蛋白H3结合到表面吸附了抗乙酰化组蛋白H3-K9抗体的微孔上.被捕获的乙酰组蛋白H3-K9被结合了一种显色剂的特异性抗体识别.乙酰化组蛋白H3-K9的量与吸光度成正比，其总量可以通过对比对照组来测定.该通用乙酰化组蛋白H3-K9定量分析试剂盒（比色法）内主要产品组份包括：C1 （10X wash buffer） C2 （antibody buffer） C3 （detection antibody， 1 mg/ml） C4 （color developer） C5 （stop solution） Standard control （100 ug/ml） 8 well sample strips （with frame） 8 well standard control strips 用户指南手册

·产品特点

1、操作十分快捷，两个半小时内可完成； 

2、独创的比色度检测方法，不接触放射性物质，无需进行电泳和色谱分析； 

3、专一性地捕获乙酰化的H3-K9，检测极限低至2 ng/孔，量程为20 ng-5ug组蛋白抽提物 /孔； 

4、附有定量检测H3-K9乙酰化的参照物； 

5、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析； 

6、操作简便、结果可靠、统一的分析条件；

·产品说明书

The EpiQuik™ Global Acetyl Histone H3-K9 Quantification Kit (Colorimetric) is a convenient package of tools that allows the experimenter to specifically measure global acetylation of histone H3-K9 colorimetrically, using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, and cultured adherent and suspension cells.  The kit has the following advantages:

• Quick and efficient procedure, which can be finished within 2.5 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
• Specifically captures acetyl H3-K9 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
• The control is conveniently included for the quantification of the amount of acetyl H3-K9.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

Background Information
Acetylation of histones, including histone H3, has been involved in the regulation of chromatin structure and recruitment of transcription factors to the gene promoters. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) play a critical role in the control of histone H3 acetylation at multiple sites. Histone H3 at lysine 9 (H3-K9) is a primary acetylated site of histone H3. Acetylation of histone H3-K9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Acetylation of histone H3-K9 is tightly involved in cell cycle regulation, cell proliferation, and apoptosis; acetylation of histone H3-K9 is also an active marker. The balance between histone H3-K9 acetylation and methylation is important for the establishment of specific chromatin structures. An imbalance in the equilibrium of histone H3 acetylation, including K9 acetylation, has been associated with tumorigenesis and cancer progression. Histone H3-K9 acetylation may be increased by inhibition of HDACs and decreased by HAT inhibition. Thus, quantitative detection of global acetyl histone H3-K9 would provide useful information for better understanding epigenetic regulation of gene activation and for developing HAT or HDAC-targeted drugs. The EpiQuik™ Global Acetyl Histone H3-K9 Quantification Kit (Colorimetric) provides a tool for measuring global acetylation of histone H3-K9.

Principle & Procedure
This kit is designed for measuring global histone H3-K9 acetylation. In an assay with this kit, the acetyl histone H3 at lysine 9 is captured to the strip wells coated with an anti-acetyl H3-K9 antibody. The captured acetyl histone H3-K9 can then be detected with a labeled detection antibody followed by a color development reagent. The ratio of acetyl H3-K9 is proportional to the intensity of absorbance. The absolute amount of acetyl H3-K9 can be quantified by comparing to the standard control.

Starting Materials
Input material should be purified histone extracts. In general, the input amount should be from 50 ng to 200 ng per well of histone extracts. 

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