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上海宇劲生物孙经理 
·产品描述
EpiQuik 通用组蛋白H3乙酰化分析试剂盒能检测通用组蛋白H3乙酰化状态，操作方便，快速稳定，比起时下其它方法更加优越安全.本试剂盒为进行检测实验提供了一整套必需的试剂和方法，即时可用.EpiQuik 通用组蛋白H3乙酰化检测试剂盒专门用来检测各种哺乳动物细胞（如新鲜和冷冻组织以及粘附培养和悬浮细胞）样品中组蛋白H3的乙酰化.
EpiQuik通用组蛋白H3乙酰化分析试剂盒专门为检测总组蛋白H3的乙酰化而设计.检测实验中，组蛋白被稳定地结合到微孔表面.乙酰化化的组蛋白H3被特异性抗体识别.乙酰化组蛋白H3可以由HRP连接的次级抗体显色系统来定量，它的量与显色程度成正比.该通用组蛋白H3乙酰化分析试剂盒 内主要产品组份包括：GF1 （10X lysis buffer） GF2 （extraction buffer） GF3 （10X Wash buffer） GF4 （histone buffer） GF5 （blocking buffer） GF6 （antibody buffer） GF7 （capture antibody， 100 ug/ml） GF8 （detection antibody， 400 ug/ml） GF9 （developing solution） GF10 （stop solution） Acetylated histone H3 control （60 ug/ml） 8 well assay strips （with frame） 用户指南手册
·产品特点
1、快速高效的检测方法，5小时内可完成；
2、独创的比色度检测方法，不接触放射性物质，无需进行电泳、抽提和色谱分析；
3、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析；
4、操作简便、结果可靠、统一的分析条件；
| Input Type: | Histone Extracts |
| Research Area: | Histone Acetylation |
| Target Application: | Amount Quantitation |
| Vessel Format: | 96-Well Plate |
| 100% Guarantee: | 6 months |
The EpiQuik™ Global Histone H3 Acetylation Assay Kit is a convenient package of tools that allows the experimenter to measure global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik™ Global Histone H3 Acetylation Assay Kit is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells. The kit has the following advantages:
Principle & Procedure
The EpiQuik™ Global Histone H3 Acetylation Assay Kit is designed for measuring global histone H3 acetylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The acetylated histone H3 can be recognized with a high-affinity antibody. The ratio or amount of acetylated histone H3 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
Product Citations
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