·产品描述

       EpiQuik通用组蛋白H3-K4二甲基定量试剂盒（比色法）是专门用来对包括新鲜和冷冻组织、贴壁培养细胞以及细胞悬浮液在内各种哺乳动物细胞（人类、鼠类等）进行组蛋白H3-K4位置二甲基化检测的工具，操作方便.

       EpiQuik通用组蛋白H3-K4二甲基定量试剂盒（比色法）专门为检测通用组蛋白H3-K4的二甲基化而设计.检测实验中，而第4位赖氨酸被二甲基化的组蛋白H3结合到表面吸附了抗二甲基组蛋白H3-K4抗体的微孔上.被捕获的二甲基组蛋白H3-K4被结合了一种显色剂的特异性抗体识别.二甲基组蛋白H3-K4的量与吸光度成正比，其总量可以通过对比对照组来测定.该通用二甲基化组蛋白H3-K4定量试剂盒（比色法）内主要产品组份包括：C1 （10X Wash Buffer） C2 （Antibody Buffer） C3 （Detection Antibody， 1 mg/ml） C4 （Color Developer） C5 （Stop Solution） Standard Control （100 ug/ml） 8-Well Sample Strips （with Frame） 8-Well Standard Control Strips 用户指南手册

·产品特点

1、操作十分快捷，两个半小时内可完成； 

2、独创的比色度检测方法，不接触放射性物质，无需进行抽提和色谱分析； 

3、专一性地捕获二甲基化的H3-K4，检测极限低至2 ng/孔，量程为20 ng-5ug组蛋白抽提物/孔； 

4、附有定量检测H3-K4二甲基化的参照物； 

5、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析； 

6、操作简便、结果可靠、统一的分析条件；

·产品说明书

The EpiQuik™ Global Di-Methyl Histone H3-K4 Quantification Kit (Colorimetric) is a convenient package of tools that allows the experimenter to specifically measure global di-methylation of histone H3-K4 colorimetrically, using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:

• Quick and efficient procedure, which can be finished within 2.5 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
• Specifically captures di-methylated H3-K4 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
• The control is conveniently included for the quantification of the amount of di-methylated H3-K4.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

Background Information
Epigenetic activation or inactivation of genes play a critical role in many important human diseases, especially in cancer. A major mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA caused by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. HMTs transfer 1-3 methyl groups from S-adenosyl-Lmethionine to the lysine and arginine residues of histone proteins. SET1, SET7/9, Ash1, ALL-1, MLL, ALR, Trx, and SMYD3 are histone methyltransferases that catalyze methylation of histone H3 at lysine 4 (H3-K4) in mammalian cells. H3-K4 di-methylation is associated with transcriptionally permissive euchromatin regions in the genome and may serve as a global epigenetic mark in euchromatin and mediates activated transcription. Increased global H3-K4 di-methylation is also found to be involved in some pathological processes such as cancer progression. The global H3- K4 di-methylation can also be changed by inhibition or activation of HMTs. Thus, quantitative detection of global di-methyl histone H3-K4 would provide useful information for better understanding epigenetic regulation of gene activation, and for developing HMT-targeted drugs. The EpiQuik™ Global Di-Methyl Histone H3-K4 Quantification Kit (Colorimetric) provides a tool for measuring global di-methylation of histone H3-K4.

Principle & Procedure
This kit is designed for measuring global histone H3-K4 di-methylation. In an assay with this kit, the di-methylated histone H3 at lysine 4 is captured to the strip wells coated with an anti-dimethyl H3-K4 antibody. The captured di-methylated histone H3-K4 can then be detected with a labeled detection antibody, followed by a color development reagent. The ratio of di-methylated H3-K4 is proportional to the intensity of absorbance. The absolute amount of di-methylated H3-K4 can be quantitated by comparing to the standard control.

Starting Materials
Input material should be purified histone extracts. In general, the input amount should be from 50 ng to 200 ng per well of histone extracts.

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