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上海宇劲生物孙经理 
·产品描述
EpiQuik通用组蛋白H3-K4甲基化分析试剂盒是专门用来对新鲜和冷冻组织、贴壁培养细胞以及细胞悬浮液进行组蛋白H3-K4位置甲基化检测的工具.
EpiQuik总组蛋白H3-K4甲基化分析试剂盒专门为检测总组蛋白H3-K4位的甲基化而设计.检测实验中，组蛋白被稳定地结合到微孔表面.甲基化的组蛋白H3-K4被特异性抗体识别.甲基化组蛋白H3-K4可以由HRP连接的次级抗体显色系统来定量，它的量与显色程度成正比.该通用组蛋白H3-K4甲基化分析试剂盒 内主要产品组份包括：GD1 （10x lysis buffer） GD2 （Extraction buffer） GD3 （10x wash buffer） GD4 （Histone buffer） GD5 （Blocking buffer） GD6 （Antibody buffer） GD7 （capture antibody， 100 ug/ml） GD8 （detection antibody， 400 ug/ml） GD9 （developing solution） GD10 （stop solution） Methylated H3-K4 control （60 ug/ml） 8 well assay strips （with frame） 用户指南手册
·产品特点
1、操作十分快捷，5小时内可完成；
2、独创的比色度检测方法，不接触放射性物质，无需进行抽提和色谱分析；
3、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析；
4、操作简便、结果可靠、统一的分析条件；
| Input Type: | Cells, Unisolated Samples |
| Research Area: | Histone Methylation |
| Target Application: | Amount Quantitation |
| Vessel Format: | 96-Well Plate |
| 100% Guarantee: | 6 months |
The EpiQuik™ Global Histone H3-K4 Methylation Assay Kit is suitable for specifically measuring global histone H3-K4 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:
Principle & Procedure
The EpiQuik™ Global H3-K4 Methylation Assay Kit is designed for measuring global histone H3-K4 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K4 can be recognized with a high-affinity antibody The ratio or amount of methylated H3-K4 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
Product Citations
Padalino G. et. al. (July 2020). Identification of 6-(piperazin-1-yl)-1,3,5-triazine as a chemical scaffold with broad anti-schistosomal activities Wellcome Open Research.
Yan N et. al. (June 2019). Aberrant Histone Methylation in Patients with Graves’ Disease International Journal of Endocrinology. 2019
Ding S et. al. (November 2017). Chronic sun exposure is associated with distinct histone acetylation changes in human skin. Br J Dermatol.
Bilan Li et. al. (March 2017). DICER1 regulates endometrial carcinoma invasion via histone acetylation and methylation Journal of Cancer. 8:933 -939.
Li B et. al. (March 2017). DICER1 regulates endometrial carcinoma invasion via histone acetylation and methylation. J Cancer. 8(6):933-939.
Zhou K et. al. (February 2017). Aberrant histone modification in CD19<sup>+</sup> B cells of patients with chronic lymphocytic leukemia. Onco Targets Ther. 10:1173-1179.
Yang Y et. al. (March 2015). The effect of mycophenolic acid on epigenetic modifications in lupus CD4<sup>+</sup>T cells. Clin Immunol.
Zhao H et. al. (January 2014). Aberrant histone methylation in the patients with immune thrombocytopenia. Platelets. 25(3):207-10.
Xiaomeng X et. al. (March 2013). Aberrant histone acetylation and methylation levels in woman with endometriosis. Arch Gynecol Obstet. 287(3):487-94.
Batra V et. al. (March 2012). Interaction between γ-radiation and dietary folate starvation metabolically reprograms global hepatic histone H3 methylation at lysine 4 and lysine 27 residues. Food Chem Toxicol. 50(3-4):464-72.
Zhao M et. al. (February 2012). Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata. Br J Dermatol. 166(2):226-73.
Chen C et. al. (October 2011). Abnormal histone acetylation and methylation levels in esophageal squamous cell carcinomas. Cancer Invest. 29(8):548-56.
Hu N et. al. (May 2008). Abnormal histone modification patterns in lupus CD4+ T cells. J Rheumatol. 35(5):804-10.
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