·产品描述

组蛋白（histones）真核生物体细胞染色质中的碱性蛋白质，含精氨酸和赖氨酸等碱性氨基酸特别多，二者加起来约为所有氨基酸残基的1/4.组蛋白与带负电荷的双螺旋DNA结合成DNA-组蛋白复合物.因氨基酸成分和分子量不同，主要分成5类.组蛋白是真核生物染色体的基本结构蛋白，是一类小分子碱性蛋白质，有五种类型：H1、H2A、H2B、H3、H4，它们富含带正电荷的碱性氨基酸，能够同DNA中带负电荷的磷酸基团相互作用.

EpiQuik总组蛋白H4定量试剂盒（比色法）是一种完整的实验组合，其含有定量组蛋白H4不同修饰水平的所有试剂，适用于人，小鼠和大鼠样本，包括新鲜或者冰冻组织，贴壁和悬浮细胞.

·产品特点

1、快速简便的操作步骤，完成实验不超过3个小时；

2、创新性的比色法，其性价比远高于放射性，电泳和色谱法；

3、特异捕获H4，最低检测极限为0.5ng/well，检测范围为50ng-1ug/孔；

4、包含未修饰的组蛋白H4作为对照；

5、可拆卸性微孔板形式，能灵活用于高通量试验；6 实验结果稳定可靠.

·产品说明书

The EpiQuik™ Total Histone H4 Quantification Kit (Colorimetric) is a complete set of essential components which enables an experimenter to colorimetrically quantify levels of histone H4 proteins independent of their modified states. It can also be used for normalizing the modified histone H4 content of samples when run in parallel with Epigentek's histone modification quantification kit series. The EpiQuik™ Total Histone H4 Quantification Kit (Colorimetric) is suitable for specifically measuring total histone H4 using human, mouse, and rat samples, including fresh and frozen tissues and cultured adherent and suspension cells.

• Quick and efficient procedure, which can be finished within 3 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
• Specifically captures histone H4 with the detection limit as low as 0.5 ng/well and detection range from 50 ng-1 µg/well of histone extracts.
• The unmodified histone H4 control is conveniently included for the quantification of the amount of total histone H4.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

Background Information
Histone H4, along with H2A, H2B, and H3 is involved in the structure of chromatin in eukaryotic cells. Histone H4 can undergo several different types of epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair. These modifications, including acetylation and methylation, occur on the N-terminal tail domains of histone H4 through catalyzation of histone modifying enzymes. This results in the remodeling of the nucleosome structure into an open conformation which is more accessible to transcription complexes. Thus, quantitative detection of various histone modifications would provide useful information for better understanding epigenetic regulation of cellular processes and for developing HMT-targeted drugs.

Principle & Procedure
In an assay with this kit, the histone H4 protein is captured to strip wells coated with an anti-histone H4 antibody. The captured histone H4 can then be detected with a detection antibody, followed by a color development reagent. The ratio of histone H4 is proportional to the intensity of absorbance by reading the optical density (OD) values in a spectrophotometer at 450 nm. The absolute amount of histone H4 can be quantified by comparing to the standard control.

Starting Materials
Input materials can be histone extracts or nuclear extracts. The amount of histone extracts for each assay can be 50 ng to 1 µg with an optimal range of 0.1 to 0.2 µg.

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