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上海宇劲生物孙经理 
·产品描述
EpiQuik总组蛋白H3定量试剂盒（比色法）提供了一整套必要试剂和方法来定量样品中组蛋白H3水平，而不依赖于其修饰状态.本试剂盒和Epigentek组蛋白修饰定量试剂盒系列联用时，还可以测定样品中被修饰的组蛋白H3水平.EpiQuik总组蛋白H3定量试剂盒（比色法）可以测定人类、小鼠以及大鼠样品（比如新鲜和冷冻组织以及黏附培养和悬浮细胞）中的总组蛋白H3含量.
EpiQuik通用组蛋白H3定量分析试剂盒（比色法）专门为定量检测通用组蛋白H3而设计，与其修饰状态无关.检测实验中，而组蛋白H3结合到表面吸附了组蛋白H3抗体的微孔上.被捕获的组蛋白H3被结合了一种显色剂的特异性抗体识别.组蛋白H3的量与吸光度成正比，其总量可以通过对比对照组来测定.该总组蛋白H3定量试剂盒（比色法）内主要产品组份包括：C1 （10X wash buffer） C2 （Antibody buffer） C3 （Detection antibody， 1mg/ml） C4 （Color developer） C5 （Stop solution） Standard control （100 ug/ml） 8 well sample strips （with frame） 8 well standard control strips 用户指南手册。
·产品特点
1、操作十分快捷，两个半小时内可完成；
2、独创的比色度检测方法，不接触放射性物质，无需进行抽提和色谱分析；
3、专一性地捕获组蛋白H3，检测极限低至10 ng/孔，量程为20 ng-1ug组蛋白抽提物/孔；
4、附有未修饰的H3作为参照物来定量检测组蛋白H3的参照物；
5、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析；
6、操作简便、结果可靠、统一的分析条件；
| Input Type: | Histone Extracts |
| Research Area: | Histone Methylation |
| Target Application: | Amount Quantitation |
| Vessel Format: | 96-Well Plate |
| 100% Guarantee: | 6 months |
The EpiQuik™ Total Histone H3 Quantification Kit (Colorimetric) is a complete set of essential components which enables the experimenter to quantify levels of histone H3 proteins independent of their modified states, and it can also be used for normalizing the modified histone H3 content of samples when run in parallel with Epigentek histone modification quantification kit series. The EpiQuik™ Total Histone H3 Quantification Kit (Colorimetric) is suitable for specifically measuring total histone H3 using human, mouse, and rat samples, including fresh and frozen tissues and cultured adherent and suspension cells. The kit has the following advantages:
Background Information
Histone H3, along with H2A, H2B, and H4, is involved in the structure of chromatin in eukaryotic cells. Histone H3 can undergo several different types of epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair. These modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occur on the N-terminal tail domains of histone H3 through catalyzing of histone modifying enzymes, which result in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, 23, and 56, and methylated at lysine 4, 9, 27, 36, and 79, and phosphorylated at ser10, ser28, Thr3, and Thr11, respectively. Thus, quantitative detection of various histone modifications would provide useful information for better understanding epigenetic regulation of cellular processes and for developing HMT-targeted drugs. Epigentek provides a series of kits used for quantifying all sites/degrees of histone H3 modification. For added convenience and more quantitative interpretation of results, we provide here the EpiQuik™ Total Histone H3 Quantification Kit (Colorimetric). This kit is designed for quantifying levels of histone H3 proteins independent of its modified state and can also be used for normalizing the modified histone H3 content of samples when run in parallel with Epigentek histone modification quantification kit series.
Principle & Procedure
This kit is designed for quantifying levels of histone h3 proteins independent of their modified states. In an assay with this kit, the histone H3 protein is captured to the strip wells coated with an anti-histone H3 antibody. The captured histone H3 can then be detected with a detection antibody followed by a color development reagent. The ratio of histone H3 is proportional to the intensity of absorbance. The absolute amount histone H3 can be quantified by comparing to the standard control.
Starting Materials
Input material should be purified histone extracts. In general, the input amount should be from 50 ng to 200 ng per well of histone extracts.
Product Citations
Parira T et. al. (September 2017). Novel detection of post-translational modifications in human monocyte-derived dendritic cells after chronic alcohol exposure: Role of inflammation regulator H4K12ac. Sci Rep. 7(1):11236.
Fujino T et. al. (November 2016). Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy. BMC Nephrol. 17(1):179.
Neri F et. al. (February 2012). Myc regulates the transcription of the PRC2 gene to control the expression of developmental genes in embryonic stem cells. Mol Cell Biol. 32(4):840-51.
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