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上海宇劲生物孙经理 
·产品描述
EpiQuik Dnmt1检测试剂盒可以检测样品中Dnmt1的含量，速度快，操作方便，比市场上同类的其它试剂盒更加优越和安全.本试剂盒即时可用，提供了检测实验所需的所有试剂和方法，无须接触放射性材料和特殊设备.试剂盒适用于新鲜组织和人类及小鼠培养细胞中Dnmt的定量检测分析.
EpiQuik的DNA甲基转移酶1检测试剂盒专为定量检测组织或细胞中的总Dnmt1而设计.在检测实验中，微孔上包被有本公司特有的Dnmt吸附基质.上样后，样品中的Dnmt1结合到基质上.被结合的Dnmt1能被特异性的Dnmt1抗体识别.可以通过类酶联免疫吸附反应后测定其比色度来定量，Dnmt1的量与显色程度成线性关系.该DNA甲基转移酶1（Dnmt1）分析试剂盒内主要产品组份包括：D1 （10X Wash Buffer） D2 （Assay Buffer） D3 （Dnmt1 Standard， 20 ug/ml） D4 （Affinity Antibody， 100 ug/ml） D5 （Detection Antibody， 200 ug/ml） D6 （Developing Solution） D7 （Stop Solution） 8-Well Assay Strips （with Frame） 用户指南手册。
·产品特点
1、操作十分快捷，3小时内可完成；
2、安全独创的比色度检测方法，不接触放射性物质，无需进行抽提和色谱分析；
3、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析；
4、操作简便、结果可靠、统一的分析条件；
The EpiQuik™ Dnmt1 Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of Dnmt1 at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt1 assay experiment without the need for electrophoresis and transfer process. The EpiQuik™ Dnmt1 Assay Kit is suitable for measuring Dnmt amounts quantitatively from fresh tissue and cultured cells of human and mouse. The kit has the following advantages:
Principle & Procedure
The EpiQuik™ Dnmt1 Assay Kit is designed for measuring total Dnmt1 amount from tissues or cells. In an assay with this kit, the unique Dnmt affinity substrate is stably coated on the strip well. The sample is added into the well and Dnmt1 contained in the sample binds to the substrate. The bound Dnmt1 can be recognized with a specific Dnmt1 antibody and colorimetrically quantified through an ELISA-like reaction. The amount of Dnmt1 is proportional to the intensity of color development.
Product Citations
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