·产品描述

   Methylamp甲基化DNA免疫沉淀（MeDIP）试剂盒提供了一整套必需的试剂和方法来富集甲基化DNA，是本公司的专利技术.试剂盒用甲基化胞嘧啶的特异性抗体与甲基化基因组DNA（meDIP）进行免疫共沉淀.富集所得到的甲基化片段可以进行标准的DNA分析.整个富集过程花费不到3小时，获得比其它试剂盒更好的富集效果.本试剂盒富集的甲基化DNA可用来进行定性和定量PCR、southern 印迹以及DNA微阵列分析.（如果您要以细胞核提取物为初始材料，请使用EpiQuik的甲基化DNA免疫共沉淀分析试剂盒）。

   Methylamp甲基化DNA免疫沉淀试剂盒中含有捕获样品中甲基化DNA所需的所有试剂.并且，本试剂盒中还有一种CHIP-级 5-甲基化抗体，以及正常小鼠的IgG阴性对照.DNA打断之后，加入微孔板中，被抗体捕获.然后DNA从复合物上解离下来，通过本公司特别设计的高速离心柱子纯化.洗脱下来的DNA可以被用于随后的各种扩增反应.该甲基化DNA免疫共沉淀试剂盒内主要产品组份包括：MC1 （Antibody Buffer） MC2 （Reaction Buffer） MC3 （Wash Buffer） MC4 （DNA Release Buffer） MC5 （Binding Buffer） MC6 （Elution Buffer） Normal Mouse IgG （1 mg/ml） Anti-5-Methylcytosine （1mg/ml） Proteinase K （10 mg/ml） 8-Well Assay Strips （with Frame） 8-Well Strip Caps F-Spin Column F-Collection Tube 用户指南手册。

·产品特点

1、高效地浓缩富集甲基化DNA，>98%； 

2、同类产品中最快速的试剂盒，3小时内完成反应； 

3、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析； 

4、附有DNA纯化柱子：节省时间和人力； 

5、操作简便、结果可靠、统一的分析条件；

·产品说明书

Please see the EpiQuik™ Methylated DNA Immunoprecipitation Kit if you are working with nuclear extract as the starting material.

The Methylamp™ Methylated DNA Capture (MeDIP) Kit is a complete set of optimized reagents to enrich and capture methylated DNA fragments in a convenient microplate-based format. The method, methylated DNA immunoprecipitation (meDIP), uses a monoclonal antibody specific to 5-methylcytosine to immunoprecipitate methylated genomic DNA. The enriched methylated fractions can then be used for gene-specific DNA methylation analysis on a genome wide scale. The Methylamp™ Methylated DNA Capture Kit is suitable for combining the specificity of enriched methylated DNA with qualitative and quantitative PCR, traditional sequencing and next-generation sequencing, DNA microarrays, as well as Southern blot. The kit has the following advantages and features:

• Highly efficient enrichment of methylated DNA: > 98%.
• Extremely fast procedure, which can be finished within 3 hours.
• Strip-well microplate format makes the assay flexible: manual or high throughput.
• Columns for DNA purification are included, saving time and reducing labor.
• Compatible with all DNA amplification-based approaches.
• Simple, reliable, and consistent assay conditions.

Background Information
MeDIP is a genome-wide, large scale technique to enrich methylated DNA by isolating methylated DNA fragments with a 5-methylcytosine antibody. This technique has played an important role in helping to identify abnormally methylated genes in diseases such as cancer. With MeDIP, the immunoprecipitated fractions of methylated DNA can be determined by PCR to evaluate the methylation state of individual regions. Additionally, the purified fractions can be input to high throughput DNA detection methods such as DNA microarrays (MeDIP-chip) and next-generation sequencing (MeDIP-seq), becoming a useful approach for methylome-level analysis and for developing comprehensive profiles of DNA methylation on a genome-wide scale.

Principle & Procedure
The most common and crucial experimental factor of MeDIP that may affect its accuracy involves the quality and cross-reactivity of the 5-methylcytosine antibody used in the procedure. Epigentek overcame such a limitation through extensive research and development to create a highly efficient MeDIP kit by applying a non-cross-reactive 5mC antibody. In the kit’s assay, DNA is sheared and then added into a microplate well or multiple wells in a high throughput format. A high quality ChIP/MeDIP-grade antibody specific to methylcytosine is then used to capture methylated DNA in the wells. The antibody-captured methylated DNA is released and is finally purified through the included spin columns. The eluted methylated DNA can now be used in various down-stream applications.

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