·产品描述

ChromaFlash染色质提取试剂盒是一组完全的、优化的试剂，可以用来从哺乳动物细胞或组织中分离染色质或是DNA-蛋白质复合物，此过程快速方便.用这个试剂盒可以用于多种染色质免疫共沉淀实验. 它同时也是Epigentek 1h ChiP 实验的推荐方法. 分离出的染色质也可以用于其他的染色质相关实验，比如体外蛋白质-DNA结合实验以及酶活实验

·产品特点

1.极度快速的方案是反应在60min内完成 

2.方便灵活，此试剂盒适用于从单层细胞、悬浮细胞或是组织中提取原始的染色质或是交联的染色质

3.未被打断的染色质使得后续实验顺利进行，包括ChIP，体外蛋白质-DNA结合实验以及酶活实验

·产品说明书

The ChromaFlash™ Chromatin Extraction Kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. It is also the recommended method for obtaining chromatin required by Epigentek’s one-hour ChIP method using the ChromaFlash™ One-Step ChIP Kit. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.

• Extremely fast procedure: the entire procedure from cell/tissue sample to ready-to-use chromatin is less than 60 minutes. 
• Convenient and flexible: the kit is suitable for preparing both native chromatin and cross-linked chromatin from monolayer or suspension cells, or from tissues. 
• Unsheared chromatin makes it customizable for various analysis workflows that require either intact or fragmented chromatin, including ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, etc.

Background Information
Chromatin immunoprecipitaton (ChIP) offers an advantageous tool for studying protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the specific sequences of a gene in living cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) techniques. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while recruitment of meH3-K9 to the promoters on a genome-wide scale can be detected by ChIP-chip. In particular, the ChIP method with specific antibodies directly against various transcriptional factors is widely demanded. 

Principle & Procedure
The ChromaFlash™ Chromatin Extraction Kit contains all reagents required for carrying out successful chromatin extraction directly from mammalian cells or tissues. Cell membranes of the sample, with or without cross-linking, are broken down using the provided lysis buffer. Chromatin or DNA-protein complex is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and stored at the appropriate temperature.

Starting Materials & Input Amount
Starting materials can include various tissue or cell samples such as cells from flask or microplate cultured cells, fresh and frozen tissues, etc. The amount of cells and tissues for each preparation can be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimal preparation, the input amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is approximately 4 ug per 106  cells or per 50 mg tissues.

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