·产品描述

FitAmp常规组织切片DNA分离试剂盒只要将本公司专利DNA分离缓冲液加到在所选显微组织区，将该区域切下来转移到一个微量离心管里.经过DNA消化缓冲液处理之后，用本公司专门设计的快速-旋转柱（Fast-Spin Column）可轻易地将DNA回收到8-20ul体系里面.所得到的DNA可以用于随后的各种实验分析.该常规组织切片DNA提取试剂盒内主要产品组份包括：S1 （DNA digestion solution） S2 （DNA digestion powder） S3 （DNA isolation buffer） S4 （DNA binding buffer） S5 （DNA elution solution） F-spin column F-collection tube 用户指南手册

·产品特点

1、步骤简单，虽然整个分离过程根据样品类型不同所需时间也不一样，但最长2小时即可完成；

2、高效专利的DNA分离技术，即使是1ng的DNA也能有效分离；

3、使用无毒性试剂，不含酚氯仿

·产品说明书

Product Overview

The FitAmp™ General Tissue Section DNA Isolation Kit is a complete set of essential components that enables researchers to efficiently isolate DNA in any targeted microscopic tissue area on a slide. It is suitable for isolating tiny amounts of DNA from microdissection samples, fresh tissue sections, formalin-fixed and paraffin-embedded tissues, plasma, serum, body fluids, etc.  Depending on sample types, the entire procedure can be completed within 2 hours.

• Fast - Entire procedure can be finished in just 2 hours with consistent isolation conditions.
• Effective -High efficiency of DNA isolation from tissue sections containing tiny amounts of DNA (as low as 1 ng).
• Reliable – Our specially designed F-Spin Columns allow for DNA to be conveniently recovered.
• Safe -Use of non-toxic reagents and no phenol chloroform.

This kit can also be employed as the initial step in DNA methylation quantitation, followed by the use of the MethylFlash Global DNA Methylation 5-mC ELISA Easy Kit, or the MethylFlash  Global DNA Methylation 5-hmC ELISA Easy Kit.

Background
Rapid and efficient preparation of DNA is crucial to a successful, DNA-based experiment. DNA samples can be prepared from cells, tissues, and body fluids, and are the most commonly used starting material for the majority of DNA methylation applications and assays. The FitAmp™ General Tissue Section DNA Isolation Kit ensures ready-to-use DNA no matter what the downstream application is.

Principle & Procedure
This kit simply applies our proprietary DNA isolation buffer to a ed microscopic tissue area. The area is removed and transferred into a tube. After treatment with DNA digestion buffer, the DNA is easily recovered in 8-20 µl by our specially designed F-Spin Column. DNA is ready for down-stream application.

Starting Materials
Input amount of tissue can be as low as 1ng with the optimal range between 10 ng and 1 µg. This kit can easily yield 1 ng to 4 µg of DNA in spin column format, which can be used for any molecular biology procedures such as PCR, restriction digestion, cloning and sequencing, etc

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