胰酶/EDTA消化液描述:
Trypsin is a serine protease produced in the pancreas. Ethylenediaminetetraacetic acid (EDTA) is a chelating agent which sequesters metal ions such as Ca+2 and Mg+2,enhancing the trypsinization reaction. 0.25% Trypsin/EDTA solution (T/E) is a sterile, phosphate and HEPES-buffered saline solution with a pH of 7.4 at room temperature. It contains 0.25% trypsin and 0.5 mM EDTA.
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说明书:
参考文献:
1.) Olsson, M., Hultman, K., Dunoyer-Geindre, S., Curtis, M.A., Faull, R.L.M., Kruithof, E.K.O. & Jern, C. (2016) Epigenetic Regulation of Tissue-Type Plasminogen Activator in Human Brain Tissue and Brain-Derived Cells Gene Regul Syst Bio. 10
2.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19