成纤维细胞培养基是专门为正常人类成纤维细胞体外培养设计的最适于其生长的培养基。它是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(2%)。该培养基缓冲体系为HEPES和重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类成纤维细胞体外培养中的增殖和生长，并为其达到最理想营养平衡状态提供数量上和质量上的保证。

         成纤维细胞培养基包含500 ml基础培养基，10ml胎牛血清(FBS,NO.0010)，5ml成纤维细胞生长添加物(FGS,NO.2352)和5 ml青霉素/链霉素溶液(P/S,NO.0503)  

产品使用说明：仅供科研研究使用

说明书：

2301.pdf

参考文献：

1.) Inoue-Mochita, M., Inoue, T., Fujimoto, T., Kameda, T., Awai-Kasaoka, N., Ohtsu, N., Kimoto, K. & Tanihara, H. (2015) p38 MAP Kinase Inhibitor Suppresses Transforming Growth Factor-Beta2-Induced Type 1 Collagen Production in Trabecular Meshwork Cells PLoS One. 10

2.) Choi, Y., Hyun, E., Seo, J., Blundell, C., Kim, H.C., Lee, E., Lee, S., Moon, A., Moon, W.K. & Huh, D. (2015) A microengineered pathophysiological model of early-stage breast cancer Lab Chip. 15

3.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) 'Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer.'

4.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) "Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer." PloS one 9: e96878.

5.) Li F, Weir MD, Chen J, Xu HHK. (2013) "Comparison of quaternary ammonium-containing with nano-silver-containing adhesive in antibacterial properties and cytotoxicity."Dent Mater. 29: 450-61.

6.) Williams M, Villarreal A, Bozhilov K, Lin S, Talbot P. (2013) "Metal and silicate particles including nanoparticles are present in electronic cigarette cartomizer fluid and aerosol." PLoS One. 8: e57987.

7.) Zhang K, Cheng L, Imazato S, Antonucci JM, Lin NJ, Lin-Gibson S, Bai Y, Xu HHK. (2013) "Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentin bond properties." J Dent. 41: 464-74.

8.) Cavet ME, Volhejn S, Harrington KL, Zhang JZ. (2013) "Anti-allergic effects of mapracorat, a novel ive glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells." Mol Vis. 19: 1515-25.

9.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) 'TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression.'

10.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) "TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression." PloS one. 8: e77366.

11.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) 'Export of microRNAs and microRNA-protective protein by mammalian cells.'

12.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) 'B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts.'

13.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) "B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J of cell physiol. 225: 251-55.

14.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) "Export of microRNAs and microRNA-protective protein by mammalian cells." Nucleic Acids Res. 38:7248-59.

15.)Ambient particulate matter attenuates Sirtuin1 and augments SREBP1-PIR axis to induce human pulmonary fibroblast inflammation: molecular mechanism of microenvironment associated with COPD,Aging (Albany NY). 2019 Jul 15; 11(13): 4654–4671.

16.)Varicella-zoster virus downregulates programmed death ligand 1 and major histocompatibility complex class I in human brain vascular adventitial fibroblasts, perineurial cells, and lung fibroblasts,J Virol. 2016 Dec 1; 90(23): 10527–10534.Published online 2016 Nov 14. Prepublished online 2016 Sep 14. doi: 10.1128/JVI.01546-16

17.)Inhibition of phosphorylated-STAT1 nuclear translocation and antiviral protein expression in human brain vascular adventitial fibroblasts infected with varicella-zoster virus,J Virol. 2014 Oct; 88(19): 11634–11637.doi: 10.1128/JVI.01945-14

18.）LTB4 activates pulmonary artery adventitial fibroblasts in pulmonary hypertension，Hypertension. 2015 Dec; 66(6): 1227–1239.Published online 2015 Oct 5. doi: 10.1161/HYPERTENSIONAHA.115.06370

19.)Tumor‐stroma interactions differentially alter drug sensitivity based on the origin of stromal cells,Mol Syst Biol. 2018 Aug; 14(8): e8322.Published online 2018 Aug 6. doi: 10.15252/msb.20188322

20.)The SOD mimic, MnTE-2-PyP, protects from chronic fibrosis and inflammation in irradiated normal pelvic tissues,Antioxidants (Basel). 2017 Dec; 6(4): 87.Published online 2017 Nov 6. doi: 10.3390/antiox6040087

21.)Cancer-cell-secreted exosomal miR-105 promotes tumour growth through the MYC-dependent metabolic reprogramming of stromal cells,Nat Cell Biol. 2018 May; 20(5): 597–609.Published online 2018 Apr 16. doi: 10.1038/s41556-018-0083-6

22.)A RASSF1A-HIF1α loop drives Warburg effect in cancer and pulmonary hypertension,Nat Commun. 2019; 10: 2130.Published online 2019 May 13. doi: 10.1038/s41467-019-10044-z