平滑肌细胞培养基是专门为正常人类平滑肌细胞体外培养设计的最适于其生长的培养基。是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(2%)。该培养基缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类平滑肌细胞体外培养中的增殖和生长，并为其达到最理想营养平衡状态。

       平滑肌细胞培养基包含500 ml基础培养基，10mll胎牛血清（FBS, Cat. No. 0010） 、5ml平滑肌细胞生长因子（SMCGS, Cat. No. 1152 ）、5 ml青霉素/链霉素溶液(P/S, Cat. No. 0503) 。

说明书：

1101.pdf

参考文献：

1.) Zhang, S., Liu, B., Fan, Z., Wang, D., Liu, Y., Li, J., Wang, N., Liu, Y. & Zhang, B. (2016) Targeted inhibition of survivin with YM155 promotes apoptosis of hypoxic human pulmonary arterial smooth muscle cells via the upregulation of voltage-dependent K+ channels Mol Med Rep. 13

2.) Wang, X.L., Zhou, Y.L., Sun, W. & Li, L. (2016) Rosuvastatin Attenuates CD40L-Induced Downregulation of Extracellular Matrix Production in Human Aortic Smooth Muscle Cells via TRAF6-JNK-NF-?B Pathway PLoS One. 11

3.) Liu, S., Guo, W., Han, X., Dai, W., Diao, Z. & Liu, W. (2016) Role of UBIAD1 in Intracellular Cholesterol Metabolism and Vascular Cell Calcification PLoS One. 11

4.) Park, S.H., Sung, Y.Y., Nho, K.J. & Kim, H.K. (2015) Protective activity ethanol extract of the fruits of Illicium verum against atherogenesis in apolipoprotein E knockout miceBMC Complement Altern Med. 15

5.) Moore-Olufemi, S.D., Olsen, A.B., Hook-Dufresne, D.M., Bandla, V. & Cox, C.S. (2015) Transforming Growth Factor-Beta 3 Alters Intestinal Smooth Muscle Function: Implications for Gastroschisis-Related Intestinal Dysfunction Dig Dis Sci. 60

6.) Li, Y., Guo, Y., Chen, Y., Wang, Y., You, Y., Yang, Q., Weng, X., Li, Q., Zhu, X., Zhou, B., Liu, X., Gong, Z. & Zhang, R. (2015) Establishment of an interleukin-1?-induced inflammation-activated endothelial cell-smooth muscle cell-mononuclear cell co-culture model and evaluation of the anti-inflammatory effects of tanshinone IIA on atherosclerosis Mol Med Rep. 12

7.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19

8.) Koo, H.J., Sohn, E.H., Pyo, S., Woo, H.G., Park, D.W., Ham, Y.M., Jang, S.A., Park, S.Y. & Kang, S.C. (2015) An ethanol root extract of Cynanchum wilfordii containing acetophenones suppresses the expression of VCAM-1 and ICAM-1 in TNF-�-stimulated human aortic smooth muscle cells through the NF-?B pathway Int J Mol Med. 35

9.) Rao VH, Kansal V, Stoupa S, Agrawal DK. (2014) 'MMP-1 and MMP-9 regulate epidermal growth factor dependent collagen loss in human carotid plaque smooth muscle cells.'

10.) Jandu SK, Webb AK, Pak A, Sevinc B, Nyhan D, Belkin AM, Flavahan NA, Berkowitz DE, Santhanam L. (2013) Nitric oxide regulates tissue transglutaminase localization and function in the vasculature. Amino Acids. 44: 261-9.

11.) Meng H, Zhang X, Lee SJ, Wang MM. (2013) "Von Willebrand Factor Inhibits Mature Smooth Muscle Gene Expression through Impairment of Notch Signaling." PLoS One. 8: e75808.

12.)Atractylodin induces myosin light chain phosphorylation and promotes gastric emptying through ghrelin receptor,Evid Based Complement Alternat Med. 2017; 2017: 2186798.Published online 2017 Aug 13. doi: 10.1155/2017/2186798