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DNA聚合酶 EconoTaq® DNA Polymerase
DNA扩增效果同常规Taq制剂一样或更好，EconoTaq的非特异性扩增的背景要低得多，EconoTaq DNA聚合酶还具有很高的批次重复性和可靠性。
EconoTaq® DNA Polymerase (with Mg++)
含MgCl2反应缓冲液，非校对聚合酶；无DNA污染；无可检测的核酸外切和内切酶活性
EconoTaq® DNA Polymerase (separate Mg++)
不含MgCl2反应缓冲液，单独MgCl2；非校对聚合酶；无DNA污染；无可检测的核酸外切和内切酶活性
| 货号 | 产品名称 | 规格 | 目录价 |
| 30031-1 | EconoTaq® DNA Polymerase (with Mg++) | 1000 U | 997 |
| 30031-2 | EconoTaq® DNA Polymerase (with Mg++) | 5000 U (5 x 1000 U) | 4749 |
| 30031-3 | EconoTaq® DNA Polymerase (with Mg++) | 10000 U (10 x 1000 U) | 9255 |
| 30032-1 | EconoTaq® DNA Polymerase (separate Mg++) | 1000 U | 997 |
| 30032-2 | EconoTaq® DNA Polymerase (separate Mg++) | 5 x 1000 U | 4749 |
| 30032-3 | EconoTaq® DNA Polymerase (separate Mg++) | 10 x 1000 U | 9255 |
说明书：
At -- for 1000 units list price (quantity discounts available), EconoTaq’s low price is coupled with high quality and performance.
QC specifications for EconoTaq are rigorous:
EconoTaq Performance
As shown in Figures 2 and 3 Lucigen’s EconoTaq DNA Polymerase performs as well as, or better than, more expensive Taq preparations from several other suppliers in routine PCR. EconoTaq is as effective in DNA amplification as another standard PCR enzyme, Tfl DNA polymerase (Figure 4). In this case, the background of non-specific amplification was much lower with EconoTaq (compare “+”and “–“ lanes for EconoTaq and Tfl in Figure 4). EconoTaq DNA Polymerase also offers high lot-to-lot reproducibility and reliability (Figures 2 and 4)
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Figure 1. High purity of EconoTaq DNA Polymerase (SDS PAGE). Lane 1, broad range molecular weight markers; Lane 2, Lucigen EconoTaq DNA Polymerase | Figure 2. Taq DNA polymerase from Promega and New England Biolabs were compared to Lucigen’s EconoTaq DNA Polymerase (2 different lots) in amplifying the ampicillin gene (0.8 kb) in a pUC19 vector. (–), no DNA. (+), DNA added (40 ng). MW, 1 kb ladder. |
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Figure 3. EconoTaq vs. AmpliTaq® (Applied Biosystems) DNA polymerase in genotyping. All PCR reactions were performed in a RoboCycler 96 (Stratagene). Hip1 genotyping was performed using the following PCR conditions: 94°C for 1min, 35 cycles of 94°C for 30sec, 62°C for 60sec, 72°C for 90sec, and 72°C for 7min. Shh, Cdo and Gas1 genotyping were performed using the following PCR conditions: 94°C for 2min, 35 cycles of 94°C for 60sec, 65°C for 60sec, 72°C for 90sec, and 72°C for 7min. All PCR reactions contained final concentrations of 1 µM of each primer, 200 µM dNTPs, 1X cresol red loading dye, and 1U of the indicated Taq polymerase. Sequences for all PCR primers have been previously published (references available). |
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Figure 4. PCR amplification was performed under standard conditions using three different lots of EconoTaq DNA Polymerase and buffer, or duplicate reactions with Tfl DNA polymerase and buffer (Promega). Reactions contained primers specific for the 16S ribosomal RNA gene, with Bacillus genomic DNA (+) or no DNA (-) as a template (1450 bp product expected). |
Please Note:
Some applications in which Lucigen's EconoTaq DNA Polymerase can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used. The PCR process is the subject of European Patent Nos. 201,184 and 200,262 owned by Hoffman-LaRoche. Those patents expired on March 28, 2006. The corresponding PCR process patents in the United States expired on March 29, 2005. It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties.
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