微信扫码
上海宇劲生物孙经理 
NxGen® phi29 DNA Polymerase 聚合酶
具有极强的链置换活性和很强的3’-5’外切校对活性。在每次反应中插入多达70000个片段，生成序列完整、高水平的DNA。
产品应用
全基因组扩增(WGA)
滚环扩增（RCA）
protein-primed DNA扩增
无细胞克隆致命的DNA
RNA-primed DNA扩增
使用挂锁探针进行原位基因分型
| 货号 | 产品名称 | 规格 | 目录价 |
| 30221-1 | phi29 DNA聚合酶 | 2000 U | 1363 |
| 30221-2 | phi29 DNA聚合酶 | 10000 U | 5543 |
说明书：
Excellent strand displacement, fidelity and speed.
phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.
 |
| An example of the savings you can expect. |
Specifications
Unit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.
Source: A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.
| Unit Concentration | 10,000 U/mL |
| Purity (SDS-PAGE) | >99% |
| SS Exonuclease | Functional |
| E. coli 16S rDNA Contamination | 100 U <10 copies |
| Endonuclease | 100 U <10% converted |
| Storage | -20&°C |
Supplied at 10,000 U/mL in 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25°C. Also supplied is 10X phi29 DNA Polymerase Buffer composed of 500 mM Tris-HCl, 100 mM (NH4)2SO4, 40 mM Dithiothreitol, 100 mM MgCl2, pH 7.5 @ 25°C. 10,000 U is supplied as 5 × 2,000 U.
购物车
