说明书：

C2LD.pdf

Application

• For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis.

Key Features

• Safe. Non-radioactive assay (cf. chromium release assay).
• Fast. The whole procedure take 20 min.
• Robust and amenable to HTS. Single reagent, "mix-incubate-measure" type assay. High-throughput assay in 96-well plates allows simultaneous processing tens of thousands of samples per day.

Method

•  OD500nm

Samples

•  Cell culture

Species

•  All

Size

•  100 tests

Shelf Life

•  12 months (-20°C)

More Details

•  Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. Cytotoxic compounds often compromise cell membrane integrity by inducing apoptosis or necrosis. LDH is a stable cytosolic enzyme that upon membrane damage is released into the cellular environment. Therefore, LDH is often measured to evaluate the presence of tissue or cell damage. The colorimetric LDH release assay is a simple and robust method to assess cytotoxic effects on cells by measuring the activity of LDH in cell culture supernatant. The assay is based on the reduction of a tetrazolium salt to a formazan dye.

·相关文献

Flage, B. (2018). Heme-induced acute lung injury by inflammasome activation in vascular endothelium (Doctoral dissertation, University of Pittsburgh). Assay: Cytotoxicity in human tissues.