说明书：

DGLDH.pdf

Application

• For quantitative determination of glutamate dehydrogenase activity.

Key Features

• Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

•  OD565nm

Samples

•  Serum, plasma, cell, tissue, agriculture etc

Species

•  All

Size

•  100 tests

Detection Limit

•  0.1 U/L

Shelf Life

•  6 months

More Details

•  GLUTAMATE DEHYDROGENASE (GLDH) is an enzyme which catalyzes the interconversion of glutamate and a-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections. BioAssay Systems' non-radioactive, colorimetric GLDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

·相关文献

Ballester-Lozano, GF et al (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr.114(5):713-26. Assay: Glutamate dehydrogenase in sea bream liver cells.

Laura BP et al. (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markersin gilthead sea bream (Sparus aurata). Aquaculture. 454 (2016) 8-18. Assay: Glutamate dehydrogenase in fish plasma.