说明书：

DIAP.pdf

Application

• For quantitative determination of albumin.

Key Features

• Sensitive and accurate. Use as little as 20 μL samples. Detection range 0.3 - 5 g/dL (45 - 750 μM) albumin in 96-well plate assay.
• Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
• Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
• Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein.

Method

•  OD610nm (BCP)

Samples

•  Serum, plasma, urine, biological preparations

Species

•  All

Size

•  250 tests

Detection Limit

•  0.3 g/dL

Shelf Life

•  12 months

More Details

•  Albumin is the most abundant plasma protein in human. It accounts for about 60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure, binding of key substances such as long-chain fatty acids, bile acids, bilirubin, haematin, calcium, magnesium. It has anti-oxidant and anticoagulant effects, and also acts as a carrier for nutritional factors and drugs, as an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition and protein-losing enteropathies. High levels are associated with dehydration. Simple, direct and automation-ready procedures for measuring albumin concentration in biological samples are becoming popular in Research and Drug Discovery. BioAssay Systems BCP albumin assay kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol purple that forms a colored complex specifically with albumin. The intensity of the color, measured at 610nm, is directly proportional to the albumin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

·相关文献

Kelly E, et al (2011). Redistribution of labile plasma zinc during mild surgical stress in the rat. Transl Res. 157(3):139-49. Assay: Albumin in rat plasma.

Olsen AS, et al (2010). Limb regeneration is impaired in an adult zebrafish model of diabetes mellitus. Wound Repair Regen. 18(5):532-42. Assay: Albumin in zebrafish blood.

Sharifuzzaman SM and Austin B (2010). Kocuria SM1 controls vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum). J Appl Microbiol. 108(6): 2162 - 2170. Assay: Albumin in fish serum.

Shin SY et al (2009). Immunological investigation in the adenoid tissues from children with chronic rhinosinusitis. Otolaryngol Head Neck Surg. 141(1):91-6. Assay: Albumin in human tissue.

Shin SY, et al (2009). IgE response to staphylococcal enterotoxins in adenoid tissues from atopic children. Laryngoscope. 119(1):171-5. Assay: Albumin in human serum.

Shin SY, et al (2009). Local production of total IgE and specific antibodies to the house dust mite in adenoid tissue. Pediatr Allergy Immunol. 20(2):134-41. Assay: Albumin in human blood.

Yung S, et al (2009). Anti-DNA antibody induction of protein kinase C phosphorylation and fibronectin synthesis in human and murine lupus and the effect of mycophenolic acid. Arthritis Rheum. 60(7):2071-82. Assay: Albumin in human urine.

Maier SM et al (2007) Proteinuria of Nonautoimmune Origin in Wild-type FVB/NJ Mice. Comp Med. 57(3) 255-266. Assay: Albumin in mouse urine and serum.