说明书：

DIPI.pdf

Application

• For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism.

Key Features

• Sensitive and accurate. Linear detection range 0.3 μM (0.0028 mg/dL) to 50 μM (0.47 mg/dL) phosphate in 96-well plate assay.
• Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
• Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate.
• Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals.

Method

•  OD620nm (malachite green)

Samples

•  Serum, urine, saliva, sweat, tissue culture, food, environment etc

Species

•  All

Size

•  500 tests

Detection Limit

•  3 μg/dL (0.3 μM)

Shelf Life

•  12 months

More Details

•  Phosphate (Pi) is one of the most important ion species in nature. Phosphate is present in all biological systems. It is a major constituent in minerals and fertilizers, and is a component of industrial wastewater. Thus accurate determination of phosphate concentration finds numerous applications in pharmacology, biomedical research, clinical chemistry, industrial process monitoring and environmental monitoring. Simple, direct and automation-ready procedures for measuring phosphate concentration in biological and environmental samples are becoming popular. BioAssay Systems phosphate assay kit is designed to measure phosphate ion directly in samples without any pretreatment. The improved Malachite Green method utilizes the malachite green dye and molybdate, which forms a stable colored complex specifically with inorganic phosphate. The intensity of the color, measured at 620nm, is directly proportional to the phosphate concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

·相关文献

Al-Saiedy, M., Gunasekara, L., Green, F., Pratt, R., Chiu, A., Yang, A. & Amrein, M. (2018). Surfactant Dysfunction in ARDS and Bronchiolitis is Repaired with Cyclodextrins. Military medicine, 183(suppl_1), 207-215. Assay: Phosphate in bovine lipid.

Dai, J., Lai, L., Tang, H., Wang, W., Wang, S., Lu, C. & Wu, Z. (2018). Streptococcus suis synthesizes deoxyadenosine and adenosine by 5'-nucleotidase to dampen host immune responses. Virulence, 9(1), 1509-1520. Assay: Phosphate in mouse blood.

Shamsuzzaman, S., Onal, M., St John, H. C., Jeffery, J. J., & Pike, J. W. (2017). Absence of the vitamin D receptor inhibits atherosclerotic plaque calcification in female hypercholesterolemic mice. Journal of cellular biochemistry, 118(5), 1050-1064. Assay: Phosphate in fish serum.

Subramaniyan, S. A., Da Rae Kang, S. A. B., Eun-So-Ri Cho, J. H., Jung, Y. C. J., Choi, Y. I., & Shim, K. S. (2016). Meat quality and physicochemical trait assessments of berkshire and commercial 3-way crossbred pigs. Korean journal for food science of animal resources, 36(5), 641. Assay: Phosphate in pig tissue.

Villa-Bellosta, R., Gonzalez-Parra, E., & Egido, J. (2016). Alkalosis and dialytic clearance of phosphate increases phosphatase activity: a hidden consequence of hemodialysis. PloS one, 11(7), e0159858. Assay: Phosphate in human plasma.

Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: phosphate in fish plasma.

Dunbar, DR. et al (2010). Transcriptional and physiological responses to chronic ACTH treatment by the mouse kidney. Physiol Genomics. 40(3):158-66. Assay: Phosphate in mouse urine.

Galitzer H, et al (2010). Parathyroid cell resistance to fibroblast growth factor 23 in secondary hyperparathyroidism of chronic kidney disease. Kidney Int.77(3):211-8. Assay: Phosphate in rat serum.

Ponda MP, et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis. 210(1):57-62. Assay: Phosphate in mouse tissue.

Xie J et al (2010). Comparison of media used to evaluate Rhizobium leguminosarum bivar viciae for phosphate-solubilizing ability. Can J Microbiol. 55(7):910-5. Assay: Phosphate in Plant tissue.

Dunbar, DR et al (2009). Transcriptional and physiological responses to chronic ACTH treatment by the mouse kidney. Physiol Genomics. 40(3):158-66. Assay: Phosphate in mouse urine.

Hildebrand, J. et al (2009) Functional and energetic characterization of P-gp-mediated doxorubicin transport in rainbow trout (Oncorhynchus mykiss) hepatocytes Comp Biochem Physiol C Toxicol Pharmacol. 149(1):65-72. Assay: Phosphate in fish hepatocyte.

O'Connor RD, et al (2009). Mecp2 deficiency decreases bone formation and reduces bone volume in a rodent model of Rett syndrome. Bone. 45(2):346-56. Assay: Phosphate in mouse tissue.

Xie J, et al (2009). Comparison of media used to evaluate Rhizobium leguminosarum bivar viciae for phosphate-solubilizing ability. Can J Microbiol. 55(7):910-5. Assay: Phosphate in plant tissue.

Abranches, J. (2008). CcpA regulates central metabolism and virulence gene expression in Streptococcus mutans. J Bacteriol. 190(7):2340-9. Assay: Phosphate in bacteria cellular ATPase.

Jubeck B, et al (2008). Promotion of articular cartilage matrix vesicle mineralization by type I collagen. Arthritis Rheum. 58(9):2809-17. Assay: Phosphate in human collagen.

Chung YI, et al (2007). Enhanced bone regeneration with BMP-2 loaded functional nanoparticle-hydrogel complex. J Control Release 121(1-2):91-9. Assay: Phosphate in rat bone.