说明书：

D2PD.pdf

Application

• For quantitative determination of peroxidase activity.

Key Features

• Fast and sensitive. Use as little as 10 μL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

•  OD570nm, or FL530/585nm

Samples

•  Biological

Species

•  All

Size

•  100 tests

Detection Limit

•  OD, FL: 4, 0.8 U/L

Shelf Life

•  12 months

More Details

•  PEROXIDASES (EC number 1.11.1.x) catalyze the following oxidation-reduction reactions:
  ROOR + electron donor (2 e-) + 2H+ → ROH + R’OH.
  For many peroxidases the optimal substrate is hydrogen peroxide (H2O2), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi and bacteria (cytochrome-c peroxidase like enzymes such a horseradish peroxidase). Simple, direct and automation-ready procedures for determining peroxidase activity find wide applications. BioAssay Systems peroxidase assay uses H2O2 and an electron donor dye that forms a pink color during the peroxidase reaction. The optical density (570nm) or fluorescence intensity (λex/em = 530/590nm) is a direct measure of the enzyme activity.

·相关文献

Ismail, I. B., Al-Busadah, K. A., & El-Bahr, S. M. (2013). Oxidative stress biomarkers and biochemical profile in broilers chicken fed zinc bacitracin and ascorbic acid under hot climate. Am J Biochem Mol Biol, 3, 202-214. Assay: Peroxidase in broiler chicks liver cells.

Hokari, R., et al. (2011). Increased expression of lipocalin-type-prostaglandin D synthase in ulcerative colitis and exacerbating role in murine colitis. Am J Physiol Gastrointest Liver Physiol 300(3):G401-8. Assay: Peroxidase in mouse.

Jawed H et al (2010) N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol 10(8):900-5 Assay: Peroxidase in rat serum.

Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164. Assay: Peroxidase in plant rice anther.

Yu CL et al (2008) A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J Bacteriol 190(2):772-6 Assay: Peroxidase in bacterial cell.