说明书：

DURE.pdf

Application

• For quantitative determination of urease activity and evaluation/screen for urease inhibitors.

Key Features

• Safe. Non-radioactive assay.
• Sensitive and accurate. As low as 0.003 U/L urease activity can be quantified.
• Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
• Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

•  OD670nm

Samples

•  Biological, environment etc

Species

•  All

Size

•  100 tests

Detection Limit

•  0.003 U/L

Shelf Life

•  6 months

More Details

•  UREASE (Amidohydrolase, EC 3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
  (NH2)2)CO + H2)O + CO2) + 2NH3)
  Many gastrointestinal or urinary tract pathogens produce urease. Thus its activity is a useful diagnostic parameter for the presence of pathogens such as Helicobacter pylori. Urease is found in bacteria, yeast, and higher plants. Urease activity is commonly determined in anaerobes of the bovine rumen, human feces and environmental samples such as soils and phytoplanktons. BioAssay Systems' Urease Assay Kit provides a very sensitive and convenient means to measure urease activity in a variety of samples including soil. In the assay, urease reacts with urea, resulting in the formation of ammonia, which is determined by the Berthelot method at 670nm. The assay is simple, sensitive, stable and high-throughput adaptable.

·相关文献

Liu, Q. W., Liu, Q. Y., Li, J. Y., Wei, L., Ren, K. K., Zhang, X. C. & Xin, H. B. (2018). Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure. Stem cell research & therapy, 9(1), 321. Assay: Urease in human urine.

Wu, D (2015). Constitutive expression of the DUR1, 2 gene in an industrial yeast strain to minimize ethyl carbamate production during Chinese rice wine fermentation. FEMS Microbiology Letters 363(1): fnv214. Assay: Urease.

Zhang, T. et al (2015). Development of an Agrobacterium-mediated transformation system for the cold-adapted fungi Pseudogymnoascus destructans and P. pannorum. Fungal Genetics and Biology 81: 73-81. Assay: urease in fungus.