说明书：

E2ND.pdf

Application

• For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.

Key Features

• Sensitive and accurate. Detection limit of 0.05 μM and linearity up to 10 μM NAD+/NADH in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

•  OD565nm

Samples

•  Cell or tissue extracts

Species

•  All

Size

•  100 tests

Detection Limit

•  0.05 μM

Shelf Life

•  6 months

More Details

•  Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NAD+/NADH concentration are very desirable. BioAssay Systems EnzyChrom™ NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by NADP+/NADPH. Our assay is a convenient method to measure NAD, NADH and their ratio.

·相关文献

Altamimi, T. R. (2018). Integrated Regulation of Cardiac Fatty Acid and Glucose Oxidation. Assay: NAD/NADH in mice heart tissue.

Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2018). STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. Redox biology, 14, 423-438. Assay: NAD/NADH in sprague dewley rats tissue.

Wang, W., Hu, Y., Wang, X., Wang, Q., & Deng, H. (2018). ROS-Mediated 15-Hydroxyprostaglandin Dehydrogenase Degradation via Cysteine Oxidation Promotes NAD+-Mediated Epithelial-Mesenchymal Transition. Cell chemical biology, 25(3), 255-261. Assay: NAD/NADH in mice tissue.

Wang, Z., Jiang, M., Guo, X., Liu, Z., & He, X. (2018). Reconstruction of metabolic module with improved promoter strength increases the productivity of 2-phenylethanol in Saccharomyces cerevisiae. Microbial cell factories, 17(1), 60. Assay: NAD/NADH in yeast cells.

He, X., Wu, C., Cui, Y., Zhu, H., Gao, Z., Li, B. & Zhao, B. (2017). The aldehyde group of gossypol induces mitochondrial apoptosis via ROS-SIRT1-p53-PUMA pathway in male germline stem cell. Oncotarget, 8(59), 100128. Assay: NAD/NADH in cotton cells.

Qiao, A., Jin, X., Pang, J., Moskophidis, D., & Mivechi, N. F. (2017). The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis. J Cell Biol, 216(3), 723-741. Assay: NAD/NADH in mice liver tissue.

Bae, S. J., Kim, S., & Hahn, J. S. (2016). Efficient production of acetoin in Saccharomyces cerevisiae by disruption of 2, 3-butanediol dehydrogenase and expression of NADH oxidase. Scientific reports, 6, 27667. Assay: NAD/NADH in yeast cells.

Bai P et al (2011). PARP-1 inhibition increases mitochondrial metabolism through SIRT1 activation. Cell Metab. 13(4):461-8. Assay: NAD/NADH in mouse cells.

Koo BS et al (2010). Improvement of coenzyme Q(10) production by increasing the NADH/NAD(+) ratio in Agrobacterium tumefaciens. Biosci Biotechnol Biochem.74(4):895-8. Assay: NAD/NADH in yeast Agrobacterium tumefaciens.

Lee M et al (2010). Depletion of GSH in glial cells induces neurotoxicity: relevance to aging and degenerative neurological diseases. FASEB J. 24(7):2533-45. Assay: NAD/NADH in human cell.

Hsu CP et al (2009). Nicotinamide phosphoribosyltransferase regulates cell survival through NAD+ synthesis in cardiac myocytes. Circ Res. 105(5):481-91. Assay: NAD/NADH in mouse heart cardiac myocytes.

Tseng HC et al (2009). Metabolic engineering of Escherichia coli for enhanced production of (R)- and (S)-3-hydroxybutyrate. Appl Environ Microbiol. 75(10):3137-45. Assay: NAD/NADH in bacteria E.coli.

Clem B,et al (2008). Small-molecule inhibition of 6-phosphofructo-2-kinase activity suppresses glycolytic flux and tumor growth. Mol Cancer Ther. 7(1):110-20. Assay: NAD/NADH in human epithelial cell.

Greenall A et al (2008). A genome wide analysis of the response to uncapped telomeres in budding yeast reveals a novel role for the NAD+ biosynthetic gene BNA2 in chromosome end protection. Genome Biol. 9(10):R146. Assay: NAD/NADH in yeast cell.

Kim Y, et al (2008). Dihydrolipoamide dehydrogenase mutation alters the NADH sensitivity of pyruvate dehydrogenase complex of Escherichia coli K-12. J Bacteriol. 190(11):3851-8. Assay: NAD/NADH in bacterial E. coli.

Olesen UH, et al (2008). Anticancer agent CHS-828 inhibits cellular synthesis of NAD. Biochem Biophys Res Commun. 367(4):799-804. Assay: NAD/NADH in human cell.

Song HK, et al (2008). Visfatin: a new player in mesangial cell physiology and diabetic nephropathy. Am J Physiol Renal Physiol. 295(5):F1485-94. Assay: NAD/NADH in human mesangial cells.

Thornburg JM et al (2008). Targeting aspartate aminotransferase in breast cancer. Breast Cancer Res. 10(5):R84. Assay: NAD/NADH in human breast adenocacinoma cell.