说明书：

E2ST.pdf

Application

• For quantitative determination of starch and evaluation of drug effects on starch metabolism.

Key Features

• Use as little as 10 μL samples. Linear detection range: 2 to 200 μg/mL starch for colorimetric assays and 0.2 to 20 μg/mL for fluorimetric assays.

Method

•  OD570nm, or FL530/585nm

Samples

•  Biological, agriculture, food etc

Species

•  All

Size

•  100 tests

Detection Limit

•  2 μg/mL

Shelf Life

•  6 months

More Details

•  STARCH , chemical formula (C6H10O5)n, is a polysaccharide carbohydrate consisting of a large number of glucose units joined together by glycosidic bonds. All plant seeds and tubers contain starch present in the form of amylose and amylopectin. Starch is the most consumed polysaccharide in the human diet. Some starches are digested very quickly, and cause a rapid and large rise in blood sugar. Others are digested more slowly, and some starch, called resistant starch, is not digested in the small intestine at all, and thus causes little or no blood sugar rise. Simple, direct and automation-ready procedures for measuring starch concentrations find wide applications in research and drug discovery. BioAssay Systems starch uses a single Working Reagent that combines the enzymatic break down of starch and the detection of glucose in one step. The color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the starch concentration in the sample. This simple convenient assay is carried out at room temperature and takes only 30 min.

·相关文献

South, P. F., Cavanagh, A. P., Liu, H. W., & Ort, D. R. (2019). Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field. Science, 363(6422), eaat9077. Assay: Starch in plant seed.

Ort, D. R., South, P. F., & Walker, B. (2018). U.S. Patent Application No. 15/913,395. Assay: Starch in Nicotiana tabacum leaf.

Wilson, C. M. (2016). Long-term impacts of invasive herbivores on tree physiology, growth, and phenology: A whole-tree perspective. Assay: Starch in hemlock root.

Gardner RD et al (2013). Comparison of CO(2) and bicarbonate as inorganic carbon sources for triacylglycerol and starch accumulation in Chlamydomonas reinhardtii. Biotechnol Bioeng. 110(1):87-96. Assay: Starch in algae Chlamydomonas reinhardtii.

Tsuge, Y., Tateno, T., Sasaki, K., Hasunuma, T., Tanaka, T., & Kondo, A. (2013). Direct production of organic acids from starch by cell surface-engineered Corynebacterium glutamicum in anaerobic conditions. AMB Express 3(1): 72. Assay: Starch in Corynebacterium glutamicum cell culture.

Boriboonkaset T et al (2012) Expression levels of some starch metabolism related genes in flag leaf of two contrasting rice genotypes exposed to salt stress. Aust. J. Crop Sci. 6(11):1579-1586. Assay: Starch in plant rice leaf.

Boriboonkaset T et al (2012) Expression levels of some starch metabolism related genes in flag leaf of two contrasting rice genotypes exposed to salt stress. Aust. J. Crop Sci. 6(11):1579-1586. Assay: Starch in Plant Rice leaf.

Zebeli Q et al (2011) Intraruminal administration of Megasphaera elsdenii modulated rumen fermentation profile in mid-lactation dairy cows. J Dairy Res 13:1-10. Assay: Starch in plant cow feed.

Iqbal S et al (2009) Feeding barley grain steeped in lactic acid modulates rumen fermentation patterns and increases milk fat content in dairy cows. J Dairy Res 92(12):6023-32. Assay: Starch in food grain starch.