说明书：

EBGL.pdf

Application

• For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.

Key Features

• Sensitive and accurate. Use as little as 20 μL samples. Linear detection range in 96-well plate: 5 to 300 μM (90 μg/dL to 5.4 mg/dL) glucose for colorimetric assays and 1 to 30 μM for fluorimetric assays.
• Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.

Method

•  OD570nm, or FL530/585nm

Samples

•  Serum, plasma, urine, saliva, milk, culture medium, food, agriculture etc

Species

•  All

Size

•  100 tests

Detection Limit

•  5 μM

Shelf Life

•  6 months

More Details

•  Glucose (C6H12O6) is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. Simple, direct and high-throughput assays for measuring glucose concentrations find wide applications in research and drug discovery. BioAssay Systems glucose assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to glucose concentration in the sample.

·相关文献

Jager, Marianne, et al (2018). Inducible knockdown of endothelial protein tyrosine phosphatase-1B promotes neointima formation in obese mice by enhancing endothelial senescence. Antioxidants & redox signaling 30.7: 927-944. Assay: Glucose in mice serum.

Das, Champak, et al (2017). Multiplexed and fully automated detection of metabolic biomarkers using microdialysis probe. Sensors and Actuators B: Chemical 238: 633-640. Assay: Glucose in glucose.

He L et al (2014). Potential biomarker of metformin action. J Endocrinol. JOE-14-0084. Assay: Glucose in mice white bloold cell.

Klippel, S et al (2012). Methyljasmonate displays in vitro and in vivo activity against multiple myeloma cells. British Journal of Haematology, 159: 340-351. Assay: Glucose in cell line.

Drew BG, et al (2011). Reconstituted high-density lipoprotein infusion modulates fatty acid metabolism in patients with type 2 diabetes mellitus. J Lipid Res. 52(3):572-81. Assay: Glucose in human plasma.

Dhar A, et al (2010). Alagebrium attenuates acute methylglyoxal-induced glucose intolerance in Sprague-Dawley rats. Br J Pharmacol.159(1):166-75. Assay: Glucose in human plasma.

Cha SH et al (2008). Differential effects of central fructose and glucose on hypothalamic malonyl-CoA and food intake. PNAS 105(44):16871-5. Assay: Glucose in mouse blood.

Wolfgang MJ et al (2007). Regulation of hypothalamic malonyl-CoA by central glucose and leptin. PNAS 104(49):19285-90. Assay: Glucose in mouse blood.

Wolfgang MJ et al (2006). The brain-specific carnitine palmitoyltransferase-1c regulates energy homeostasis. PNAS 103(19):7282-7. Assay: Glucose in mouse plasma.