说明书：

ECIT.pdf

Application

• For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.

Key Features

• Fast and sensitive. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

•  OD570nm, or FL530/585nm

Samples

•  Serum, plasma, agriculture etc

Species

•  All

Size

•  100 tests

Detection Limit

•  OD, FL: 4, 0.5 μM.

Shelf Life

•  6 months

More Details

•  CITRATE is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. BioAssay Systems' Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample.

·相关文献

Fu, Xuekun, et al (2018). Runx2/Osterix and zinc uptake synergize to orchestrate osteogenic differentiation and citrate containing bone apatite formation. Advanced Science 5.4: 1700755. Assay: Citrate in mice cells.

Trivedi, Amit Kumar, et al. (2015). Adaptation of oxidative phosphorylation to photoperiod-induced seasonal metabolic states in migratory songbirds. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 184): 34-40. Assay: Citrate in bird plasma.