说明书：

EDLC.pdf

Application

• For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

Key Features

• Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

•  OD565 nm

Samples

•  Serum, plasma, cell culture media etc

Species

•  All

Size

•  100 tests

Detection Limit

•  0.05 mM

Shelf Life

•  6 months

More Details

•  Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

·相关文献

Aranda-Diaz, Andres, et al (2019). Bacterial interspecies interactions modulate pH-mediated antibiotic tolerance in a model gut microbiota. bioRxiv: 538132. Assay: D-Lactate in bacteria culture.

Kwan, Billy KY, et al (2017). Marine microalgae as dietary supplements in the culture of juvenile Chinese horseshoe crabs, Tachypleus tridentatus (Xiphosura). Aquaculture research 48.7: 3910-3924. Assay: D-Lactate in horseshoe crabs plasma.

Marcu, R et al (2014). Mitochondrial Matrix Ca2 Accumulation Regulates Cytosolic NAD/NADH Metabolism, Protein Acetylation, and Sirtuin Expression. Mol Cell Biol. 34(15):2890-902. Assay: D-Lactate in human endothelial cells.

Raykov, Z et al (2014). Myo-inositol trispy rophosphate-mediated hypoxia reversioncontr ols pancreatic cancer in rodents and enhances gemcitabineefficacy. Int J Cancer. 134(11):2572-82. Assay: D-Lactate in pancreatic tumor cells in rats and mice.

Raykov, Z et al (2014). Myo-inositol trispyrophosphate-mediated hypoxia reversion controls pancreatic cancer in rodents and enhances gemcitabineefficacy. Int J Cancer. 134(11):2572-82. Assay: D-Lactate in rats and mice pancreatic tumor cells.

Guzman, FJ, et al (2013). Temporal relationship of serum markers and tissue damage during acute intestinal ischemia/reperfusion. Clinics (Sao Paulo). 68(7):1034-8. Assay: D-Lactate in rat serum.

Witkin SS., et al (2013). Influence of vaginal bacteria and D- and L-lactic acid isomers on vaginal extracellular matrix metalloproteinase inducer: implications for protection against upper genital tract infections. MBio. 6;4(4). pii: e00460-13. Assay: D-Lactate in human vaginal secretions.

Gigante A et al (2012) D-Lactic acidosis 25 years after bariatric surgery due to Salmonella enteritidis. Nutrition 28(1):108-11. Assay: D-Lactate in human serum.

Kurundkar, A.R., et al. (2010). Extracorporeal membrane oxygenation causes loss of intestinal epithelial barrier in the newborn piglet. Pediatr Res 68(2):128-33. Assay: D-Lactate in pig plasma.