说明书：

EFLLC.pdf

Application

• For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.

Key Features

• Sensitive and accurate. Detection limit of 1 μM and linearity up to 50 μM L-lactate in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

•  FL530/585 nm

Samples

•  Serum, plasma, cell culture media etc

Species

•  All

Size

•  100 tests

Detection Limit

•  1 μM

Shelf Life

•  6 months

More Details

•  LACTATE is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems EnzyFluo™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the lactate concentration in the sample.

·相关文献

Rudnicki, Martina, et al (2018). Endothelial-specific FoxO1 depletion prevents obesity-related disorders by increasing vascular metabolism and growth. eLife 7: e39780. Assay: L-Lactate in mice serum.

Konrad, Csaba, et al (2017). Fibroblast bioenergetics to classify amyotrophic lateral sclerosis patients. Molecular neurodegeneration 12.1: 76. Assay: L-Lactate in human cells.

Palomino, Nahui, et al (2017). Vaginal Lactobacillus inhibits HIV-1 replication in human tissues ex vivo. Frontiers in microbiology 8: 906. Assay: L-Lactate in Lactobacillus culture.

Mongersun, Amy, et al. (2016). Droplet Microfluidic Platform for the Determination of Single-Cell Lactate Release. Analytical chemistry 88.6: 3257-3263. Assay: L-Lactate in unknown cancer cells.

Saville, Renee M., et al (2016). Compositions and methods for biological production of lactate from C1 compounds using lactate dehydrogenase transformants. U.S. Patent Application No. 14/898,948. Assay: L-Lactate in microorganism culture.