说明书：

EGAL.pdf

Application

• For quantitative determination of galactose and its metabolism.

Key Features

• Use as little as 20 μL samples. Linear detection range in 96-well plate: 10 to 1000 μM galactose for colorimetric assays and 10 to 100 μM for fluorimetric assays.

Method

•  OD570nm, or FL530/585nm

Samples

•  Serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture etc

Species

•  All

Size

•  100 tests

Detection Limit

•  10 μM

Shelf Life

•  12 months

More Details

•  Galactose (C6H12O6) is a monosaccharide that is found in dairy products, sugar beets, gums and mucilages. It is also synthesized in mammals, where it forms part of glycolipids and glycoproteins in several tissues. It forms the disaccharide lactose when combined with glucose. Simple, direct and high-throughput assays for galactose determination find wide applications. BioAssay Systems assay uses specific enzyme-coupled reactions to form a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the galactose concentration in the sample.

·相关文献

Shi, Jia, Yu Fu, and Xin-Huai Zhao (2018). Effects of Maillard-type caseinate glycation on the preventive action of caseinate digests in acrylamide-induced intestinal barrier dysfunction in IEC-6 cells. RSC advances 8.66: 38036-38046. Assay: Galactose in caseinate.

Sun, Ya-ting, et al. (2013). Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose. Biochemical Engineering Journal 81: 126-135. Assay: Galactose in human culture medium.

Ya-ting, S et al (2013). Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose. Biochemical Engineering Journal 81: 126-135. Assay: Galactose in cells.