说明书：

EGLT.pdf

Application

• For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism.

Key Features

• Sensitive and accurate. Detection limit of 50 μM, linearity up to 2.5 mM glutamate in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

•  OD565nm

Samples

•  Serum, plasma, urine, cell, tissue etc

Species

•  All

Size

•  100 tests

Detection Limit

•  50 μM

Shelf Life

•  6 months

More Details

•  Glutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism and Alzheimer’s disease. Glutamate is also widely used as a flavor enhancer in the food industry. Simple, direct and automation-ready procedures for measuring glutamate concentration are very desirable. BioAssay Systems EnzyChrom™ glutamate assay kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample.

·相关文献

Kansakar, U. (2019). Development of an Astrocyte/Glioma Co-Culture System for Measuring Cellular Dynamics. Assay: Glutamate in rat cells.

Izrael, Michal, et al (2018). Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1 G93A and NSG animal models. Stem cell research & therapy 9.1: 152. Assay: Glutamate in human cells.

Das, Champak, et al (2017). Multiplexed and fully automated detection of metabolic biomarkers using microdialysis probe. Sensors and Actuators B: Chemical 238: 633-640. Assay: Glutamate in glucose.

Swiercz, Rafal, et al (2017). Loss of expression of the recycling receptor, FcRn, promotes tumor cell growth by increasing albumin consumption. Oncotarget 8.2: 3528-3541. Assay: Glutamate in human cells.

Izrael, Michal, et al (2016). Directed differentiation of astrocytes from human pluripotent stem cells for use in drug screening and the treatment of amyotrophic laterial sclerosis (als). U.S. Patent Application No. 15/025,946. Assay: Glutamate in human cells.

Kahlert, Ulf D., et al (2016). Alterations in cellular metabolome after pharmacological inhibition of N otch in glioblastoma cells. International journal of cancer 138.5: 1246-1255. Assay: Glutamate in human cells.

Wray, Robin, et a (2016). Dihydrostreptomycin directly binds to, modulates, and passes through the MscL channel pore. PLoS biology 14.6: e1002473. Assay: Glutamate in E. coli cells.

Mesci, P et al (2015). System xC- is a mediator of microglial function and its deletion slows symptoms in amyotrophic lateral sclerosis mice. Brain. 138(Pt 1):53-68. Assay: Glutamate in mice spinal cord tissue.

Iscla, I et al (2014). Streptomycin potency is dependent on MscL channel expression. Nature Communications. 5:4891. Assay: Glutamate in E. coli vector.

Ramos, C et al (2012). Native Presynaptic Metabotropic Glutamate Receptor 4 (mGluR4) Interacts with Exocytosis Proteins in rat Cerebellum. Journal of Biological Chemistry 287(24):20176-86. Assay: Glutamate in rat protein lysate.

Liang, Y., et al. (2011). Additive effect of tetramethylpyrazine and deferoxamine in the treatment of spinal cord injury caused by aortic cross-clamping in rats. Spinal Cord 49(2):302-6. Assay: Glutamate in rat.

Rink, C., et al (2011). Oxygen-inducible glutamate oxaloacetate transaminase as protective switch transforming neurotoxic glutamate to metabolic fuel during acute ischemic stroke. Antioxid Redox Signal 14(10):1777-85. Assay: Glutamate in rat brain tissue.

Yang, LM and Blount, P. (2011). Manipulating the permeation of charged compounds through the MscL nanovalve. FASEB J 25(1):428-34. Assay: Glutamate in E. coli.