说明书：

EGLY.pdf

Application

• For quantitative determination of glycerol and evaluation of drug effects on glycerol metabolism.

Key Features

• Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 10 to 1000 μM (92 μg/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 μM for fluorimetric assays.
• Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays.
• Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.

Method

•  OD570nm, or FL530/585nm

Samples

•  Biological, food, beverage etc

Species

•  All

Size

•  200 tests

Detection Limit

•  2 μM

Shelf Life

•  12 months

More Details

•  Glycerol [Glycerin or Glycerine, C3H5 (OH) 3] is widely used in foods, beverages and pharmaceutical formulations. It is also a main by-product of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications. BioAssay Systems glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to glycerol concentration in the sample.

·相关文献

Castillo, F., Gonzalez, D. R., & Moore-Carrasco, R. (2019). Effects of Phaseolus vulgaris Extract on Lipolytic Activity and Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes: A Strategy to Prevent Obesity. Journal of Nutrition and Metabolism, 2019. Assay: Glycerol in mouse cells.

Pan, Y., Zhao, W., Zhao, D., Wang, C., Yu, N., An, T. & Gu, Y. (2018). Salvianolic acid B improves mitochondrial function in 3T3-L1 adipocytes through a pathway involving PPARgamma coactivator-1alpha (PGC-1alpha). Frontiers in pharmacology, 9:671. Assay: Glycerol in mouse cells.

Sharma, V., Smolin, J., Nayak, J., Ayala, J. E., Scott, D. A., Peterson, S. N., & Freeze, H. H. (2018). Mannose Alters Gut Microbiome, Prevents Diet-Induced Obesity, and Improves Host Metabolism. Cell reports, 24(12), 3087-3098. Assay: Glycerol in mice serum.

Zhao, Y., Li, X., Yang, L., Eckel-Mahan, K., Tong, Q., Gu, X. & Sun, K. (2018). Transient overexpression of VEGF-A in adipose tissue promotes energy expenditure via activation of the sympathetic nervous system. Molecular and cellular biology, MCB-00242. Assay: Glycerol in mice tissues.

Loyola-Machado, A. C., Azevedo-Martins, A. C., Catta-Preta, C. M. C., de Souza, W., Galina, A., & Motta, M. C. M. (2017). The symbiotic bacterium fuels the energy metabolism of the host trypanosomatid Strigomonas culicis. Protist, 168(2), 253-269. Assay: Glycerol in bacteria (S. culicis) cells.

Allsopp, P., Crowe, W., Bahar, B., Harnedy, P. A., Brown, E. S., Taylor, S. S. & Strain, C. R. (2016). The effect of consuming Palmaria palmata-enriched bread on inflammatory markers, antioxidant status, lipid profile and thyroid function in a randomised placebo-controlled intervention trial in healthy adults. European journal of nutrition, 55(5), 1951-1962. Assay: Glycerol in mouse cells.

Burch, T. A. (2016). The Compatible Solute Glycerol as a Photosynthetic Sink and Energy Carrier in Freshwater and Marine Chlamydomonas. Assay: Glycerol in Algae cells.

Coughlin, David J., et al (2016). Elevated osmolytes in rainbow smelt: the effects of urea, glycerol and trimethylamine oxide on muscle contractile properties. Journal of Experimental Biology 219.7: 1014-1021. Assay: Glycerol in fish tissues.

Eastwood, L., Leterme, P., & Beaulieu, A. D. (2016). Body fat mobilization during lactation in high-producing sows fed varied omega-6 to omega-3 fatty acid ratios. Canadian Journal of Animal Science, 96(1), 69-78. Assay: Glycerol in sow serum.

Gallagher, H. L., Boule, M. A., & Zonfrelli, T. A. (2016). An Analytical Comparison of Ethanolysis and Methanolysis Kinetics and the Refined Biodiesel Product. Assay: Glycerol in alcohol and canola oil mixture.

Vadassery, J., Oelmuller, R., Dua, M., Johri, A. K., Nevo, E., Jogawat, A., & Verma, N. (2016). PiHOG1, a stress regulator MAP kinase from the root endophyte fungus Piriformospora indica, confers salinity stress tolerance in rice plants. Sci Rep. 6:36765. Assay: Glycerol in yeast cells.

Zhao, Y., Gu, X., Zhang, N., Kolonin, M. G., An, Z., & Sun, K. (2016). Divergent functions of endotrophin on different cell populations in adipose tissue. American Journal of Physiology-Endocrinology and Metabolism, 311(6), E952-E963. Assay: Glycerol in mouse cells.

Calamita G et al (2012). Biophysical assessment of aquaporin-9 as principal facilitative pathway in mouse liver import of glucogenetic glycerol. Biol Cell 104(6):342-51. Assay: Glycerol in mouse plasma.

Bahar, B., et al. (2011). A potential role of IL-6 in the chito-oligosaccharide-mediated inhibition of adipogenesis. Br J Nutr. 106(8): 1142-53. Assay: Glycerol in mouse 3T3-L1 cells.

Drew, B.G., et al. (2011). Reconstituted high-density lipoprotein infusion modulates fatty acid metabolism in patients with type 2 diabetes mellitus. J Lipid Res 52(3):572-81. Assay: Glycerol in human plasma.

Pifferi, F., et al. (2011). Mild experimental ketosis increases brain uptake of 11C-acetoacetate and 18F-fluorodeoxyglucose: a dual-tracer PET imaging study in rats. Nutr Neurosci 14(2):51-8. Assay: Glycerol in rat plasma.

Wachtler, B., et al. (2011). From attachment to damage: defined genes of Candida albicans mediate adhesion, invasion and damage during interaction with oral epithelial cells. PLoS One 6(2):e17046. Assay: Glycerol in Candida albicans fungus.

Lung, M., et al. (2009). Diacylglycerol kinase epsilon is selective for both acyl chains of phosphatidic acid or diacylglycerol. J Biol Chem 284(45):31062-73. Assay: Glycerol in diacylglycerol.