说明书：

EGTT.pdf

Application

• For quantitative determination of reduced and oxidized glutathione (GSH/GSSG).

Key Features

• Sensitive and accurate. Linear detection range 0.01-3 µM GSH equivalents with a detection limit of 10 nM GSH equivalents.

Method

•  OD412nm

Samples

•  Whole blood, plasma, serum, urine, tissue and cell extracts

Species

•  All

Size

•  100 tests

Detection Limit

•  10 nM

Shelf Life

•  6 months

More Details

•  Glutathione , a tripeptide of glycine, glutamic acid and cysteine, is one of the key antioxidants involved in protecting cells from damages by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG. BioAssay Systems GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density,measured at 412 nm, is directly proportional to glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol which first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.

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Lin, T. A., Ke, B. J., Cheng, C. S., Wang, J. J., Wei, B. L., & Lee, C. L. (2019). Red Quinoa Bran Extracts Protects against Carbon Tetrachloride-Induced Liver Injury and Fibrosis in Mice via Activation of Antioxidative Enzyme Systems and Blocking TGF-beta1 Pathway. Nutrients, 11(2), 395. Assay: GSH/GSSG in mice tissues.

Kim, K. H., Kim, E. Y., Lee, S. Y., Ko, J. J., & Lee, K. A. (2018). Oocyte Cytoplasmic Gas6 and Heparan Sulfate (HS) are Required to Establish the Open Chromatin State in Nuclei During Remodeling and Reprogramming. Cellular Physiology and Biochemistry, 45(1), 37-53. Assay: GSH/GSSG in mice cells.

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Di Maro, G., et al. (2014). Anterior gradient protein 2 promotes survival, migration and invasion of papillary thyroid carcinoma cells. Molecular cancer, 13(1), 1. Assay: GSH/GSSG in human tissues.

Muller, S., et al. (2014). Age-dependent effects of UCP2 deficiency on experimental acute pancreatitis in mice. PloS one, 9(4), e94494. Assay: GSH/GSSG in mice serum.