说明书：

EIVT.pdf

Application

• For quantitative determination of invertase/sucrase activity.

Key Features

• Safe. Non-radioactive assay.
• Sensitive and accurate. As low as 0.007 U/L invertase activity can be quantified.
• Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
• Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

•  OD570nm, or FL530/585nm

Samples

•  Biological, environment (soil) etc

Species

•  All

Size

•  100 tests

Detection Limit

•  0.007 U/L

Shelf Life

•  6 months

More Details

•  Invertase (b-fructofuranosidase, EC 3.2.1.26) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertases cleave at the O-C(fructose) bond, whereas a related enzyme sucrase (EC 3.2.1.48) cleaves at the O-C(glucose) bond. A wide range of microorganisms produce invertase and can, thus, utilize sucrose as a nutrient. Invertase assay finds wide applications in environmental (e.g. soil), agricultural and food (confectionery) industry. BioAssay Systems Invertase Assay Kit provides a convenient and ultra-sensitive colorimetric and fluorimetric means to measure invertase activity. In the assay, invertase cleaves sucrose, resulting in the formation of fructose and glucose, which is determined by a colorimetric (570nm) or fluorimetric method (λex/em = 530/585nm). The assay is simple, sensitive, stable and high-throughput adaptable.

·相关文献

Chua, L. S., et al (2014). Effect of thermal treatment on the biochemical composition of tropical honey samples. International Food Research Journal 21(2): 773-778. Assay: Invertase in honey.

Chua, L. S., et al.(2014). Effect of thermal treatment on the biochemical composition of tropical honey samples. International Food Research Journal 21(2): 773-778. Assay: Invertase/Sucrase in Honey.

Ciesielska, K et al (2014). Exoproteome analysis of Starmerella bombicola results in the discovery of an esterase required for lactonization of sophorolipids. Journal of Proteomics(98):159-174. Assay: Invertase in S. bombicola protein extract.