说明书：

EPPD.pdf

Application

• For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

Key Features

• Sensitive. Use 10 μL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.
• Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.

Method

•  OD570nm, or FL530/585nm

Samples

•  Biological

Species

•  All

Size

•  100 tests

Detection Limit

•  0.04 U/L

Shelf Life

•  6 months

More Details

•  Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. BioAssay Systems method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.

·相关文献

Zhu X et al (2014). The role of phospholipase D1 in liver fibrosis induced by dimethylnitrosamine in vivo. Dig Dis Sci. 59(8):1779-88. Assay: Phospholipase D in rat tissue.