说明书：

ESUC.pdf

Application

• For quantitative determination of sucrose.

Key Features

• No interference by glucose.
• Use 20 μL samples. Linear detection range: 17 to 2000 μM sucrose.

Method

•  OD565nm

Samples

•  Serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture etc

Species

•  All

Size

•  100 tests

Detection Limit

•  17 μM

Shelf Life

•  6 months

More Details

•  Sucrose (C12H22O11) is a disaccharide of glucose and fructose with a 1,2-glycosidic linkage. It is the most common food sweetener and the most important sugar in plants. In mammals, sucrose is readily digested in the stomach into glucose and fructose, which are rapidly absorbed into the bloodstream in the small intestine. Simple, direct and high-throughput assays for measuring sucrose concentrations find wide applications. BioAssay Systems improved assay uses invertase to digest sucrose into fructose and glucose. The resulting fructose is then quantified using our fructose assay reagent. The measured color intensity at 565nm is directly proportional to the sucrose concentration in the sample.

·相关文献

Chen, X., Zhu, M., Gu, F., Liu, M., Zhang, Y., Xing, Y., & He, G. (2018). Identification and gene fine mapping of starch accumulation and early senescent leaf mutant esl10 in rice. Crop Science, 58(1), 204-217. Assay: Sucrose in microbe leaves.

Huang, J., Yan, M., Zhu, X., Zhang, T., Shen, W., Yu, P., & He, G. (2018). Gene mapping of starch accumulation and premature leaf senescence in the ossac3 mutant of rice. Euphytica, 214(10), 177. Assay: Sucrose in e. coli leaves.

Inukai, T. (2017). Differential Regulation of Starch-synthetic Gene Expression in Endosperm Between Indica and Japonica Rice Cultivars. Rice, 10(1), 7. Assay: Sucrose in rice karyopsis tissue.

Nakajima, Y., Maeda, K., Jin, Q., Takahashi-Ando, N., Kanamaru, K., Kobayashi, T., & Kimura, M. (2016). Oligosaccharides containing an alpha-( - 2)(glucosyl/xylosyl)-fructosyl linkage as inducer molecules of trichothecene biosynthesis for Fusarium graminearum. International journal of food microbiology, 238, 215-221. Assay: Sucrose in Fusarium graminearum cell lysate.