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货号:AAH-CYT-G2000-4


产品描述:Human Cytokine Array G2000


产品类型:Antibody Array


产品规格:4 samples/kit

名称:Human Cytokine Array G2000
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货号:AAH-CYT-G2000-4


产品描述:Human Cytokine Array G2000


产品类型:Antibody Array


产品规格:4 samples/kit


RayBio® G-Series Human Cytokine Antibody Array 2000 Kit. A combination of Human Cytokine Antibody Array G6, G7, & G8. Detects 174 Human Cytokines. Suitable for all liquid sample types.


Antigen Information

Gene Symbols:
ADIPOQ | AGRP | ALCAM | ANG | ANGPT2 | AREG | AXL | BDNF | BMP4 | BMP5   View more

Assay Format

Number of Targets Detected:
174
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
Activin A
AgRP
ALCAM
(CD166)
Amphiregulin
Angiogenin
Angiopoietin-2
Axl
CD80
(B7-1)
BDNF
bFGF
BLC
(CXCL13)
BMP-4
BMP-5
BMP-6
BMP-7
beta-NGF
Betacellulin
(BTC)
Cardiotrophin-1
(CT-1)
CCL28
(MEC)
CD14
Ck beta 8-1
(CCL23)
CNTF
CTACK
(CCL27)
CXCL16
DR6
(TNFRSF21)
Dtk
EGF
EGFR
ENA-78
(CXCL5)
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
ErbB3
E-Selectin
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GCSF
GDNF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
ICAM-2
(CD102)
ICAM-3
(CD50)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IGF-2
IL-1 R2
IL-1 R4
(ST2)
IL-1 R1
IL-10
IL-10 R beta
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-13 R alpha 2
IL-15
IL-16
IL-17A
IL-18 BP alpha
IL-18 R beta
(AcPL)
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R beta
(CD122)
IL-2 R gamma
(Common gamma Chain)
IL-2 R alpha
IL-21 R
IL-3
IL-4
IL-5
IL-5 R alpha
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
IL-9
IP-10
(CXCL10)
I-TAC
(CXCL11)
LAP/TGF beta 1
Leptin
Leptin R
LIF
Light
(TNFSF14)
L-Selectin
(CD62L)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
M-CSF R
MDC
(CCL22)
MIF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MMP-1
MMP-13
MMP-3
MMP-9
MPIF-1
(CCL23)
MSP alpha/beta
NAP-2
(PPBP/CXCL7)
NGFR
(TNFRSF16)
NT-3
NT-4
Oncostatin M
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PDGF-AA
PDGF R alpha
PDGF R beta
PDGF-AB
PDGF-BB
PECAM-1
(CD31)
PLGF
Prolactin
RANTES
(CCL5)
SCF
SCF R
(CD117/c-kit)
SDF-1 alpha
(CXCL12 alpha)
SDF-1 beta
(CXCL12 beta)
gp130
Siglec-5
(CD170)
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TARC
(CCL17)
TECK
(CCL25)
TGF beta 2
TGF alpha
TGF beta 3
TGF beta 1
Thrombopoietin
(TPO)
Tie-1
Tie-2
TIMP-1
TIMP-2
TIMP-4
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VE-Cadherin
(CDH5)
VEGF-A
VEGFR2
VEGFR3
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array G2000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.