·产品描述

EpiQuik总组蛋白提取试剂盒只需要用本公司的专利组蛋白分离缓冲液加到细胞/组织样品中.先后加入细胞预溶解剂、细胞溶解剂以及平衡缓冲液，总组蛋白即可被提取出来并立即用于随后的各种实验分析或在适当条件下储存起来.该总组蛋白提取试剂盒内主要产品组份包括：10X Pre-Lysis Buffer Lysis Buffer Balance Buffer DTT Solution 用户指南手册

·产品特点

1、操作十分快捷，1小时内可完成；

2、应用广泛，可用于多种样本的核蛋白提取

·产品说明书

Product Overview

The EpiQuik™ Total Histone Extraction Kit is a complete set of optimized buffers and reagents for extracting total core histone proteins (H2A, H2B, H3, and H4) from mammalian cells or tissues in a simple 60 minute procedure. The post-translational modifications (PTM) in the histone extracts are kept intact and thus can be used with Epigentek's histone modification assay kits  or in a variety of downstream applications for histone methylation, acetylation, phosphorylation, sumoylation, ubiquitination, citrullination, and ADP-ribosylation  studies.

• Pre-optimized and simple 1 hour protocol.
• Conveniently includes all essential reagents to carry out a histone extraction.
• Standardized procedure for reproducible results.
• Extracts a high yield of total core histones from as little as 1 mg of tissues.
• Post-translational modifications are kept intact.
• Does not affect histone modification status or levels.

Background Information
Histones are the chief protein components of chromatin in biology. They act as spools around which DNA winds, and also play a role in gene regulation. The core histones include H2A, H2B, H3, and H4. Histones undergo post-translational modifications, which alter their interaction with DNA and nuclear proteins. The H3 and H4 histones have long tails protruding from the nucleosome, which can be covalently modified at several places (i.e., methylation, acetylation, phosphorylation, etc.). Histone modifications act in diverse biological processes such as gene regulation, DNA repair and chromosome condensation (mitosis). Combinations of modifications are thought to constitute a "histone code" and an understanding of this code may help to answer questions about human development and disease.

Principle & Procedure
The EpiQuik™ Total Histone Extraction Kit simply applies our proprietary histone isolation buffers to cell or tissue samples. After treatment with pre-lysis, lysis, and balance buffers, the total core histones are easily extracted for immediate use or storage under proper conditions.

Starting Materials
The minimal amount of starting materials can be as low as 105 cells or 1 mg tissue. For the best results, the cell number should be greater than 106 cells or the tissue amount should be greater than 10 mg. A total of 100 standard extractions (using 107 cells or 100 mg tissues per extraction) can be performed with this kit. Yield of the total histone proteins is approximately 0.4 mg per 107 cells or 100 mg of tissue. The yield may vary depending on cell or tissue type.

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