| 品牌 | 货号 | 产品名称 | 规格 |
| Enzo | ENZ-51031-0050 | CYTO-ID Autophagy detection kit CYTO-ID自噬检测试剂盒 | 50 tests |
| Enzo | ENZ-51031-K200 | CYTO-ID Autophagy detection kit CYTO-ID自噬检测试剂盒 | 200 tests |
产品说明书：
ENZ-51031_insert.pdf
FAQ：
ENZ-51031FAQ.pdf
·特点
·无需转染，定量监测活细胞中的自噬情况
·免去LC3-GFP转染所需的时间和精力以及转染效率的验证
·含有特殊基团的专利染料选择性地染色自噬小泡
·试剂盒中包含已知活性的自噬抑制剂与激活剂
·快速量化原生异质性细胞群中的自噬
·选择地全方位染色，可检测和区分自噬流（autophagic flux）和自噬溶酶体的积累
·不染溶酶体，减少其他染料的背景干扰
·便于高通量筛选自噬激活剂和抑制剂
·原理
该产品所用探针是阳离子两亲性示踪剂（CAT）染料，以类似诱导磷脂药物的方式迅速进入到细胞中。染料的功能基团能够选择性标记与自噬通路相关的小泡，而不会在溶酶体中聚集。
胞内物质被扩大的膜囊包裹，吞噬泡形成双层膜囊泡，成为自噬体。自噬体外膜随后与溶酶体融合，和内部物质被自噬性溶酶体降解。自噬的各种调节因子也被描绘于图中。
自噬原理图
·应用
省时省力，无需转染即可自快速全面的标记自噬小泡
为了演示CYTO-ID@Green detection reagent的优势，先用RFP-LC3表达载体转染HeLa细胞，10uM的Tamoxifen处理过夜，然后用CYTO-ID@绿色检测试剂染色。LC3转染法需要过夜，而CYTO-ID@绿色检测试剂的方法在15-30分钟内标记细胞达100%。
图A：绿信号显示自噬小泡的CYTO-ID@染绿；图B：成功转细胞的RFP-LC3表达（红色）；图C：自噬体的特异性标记LC3，与CYTO-ID@绿色染料标记的小泡共定位的复合图像。
无转染的自噬检测
Hela细胞进行饥饿与恢复，然后使用CYTO-ID@
Green detection reagent标记。染料可以明确检测和量化自噬及与自噬诱导相关的自噬小泡前体。
图1A：稳定表达GFP-LC3的CHO细胞，结果显示对照组与饥饿的细胞群的相对较差的基线分离，自噬难以量化。
图1B:CYTO-ID@Autophagy Detection Kit特异性标记LC3蛋白依赖的自噬小泡，免去转染操作。
自噬积累和自噬流的可视化
可用荧光显微镜观察CYTO-ID@自噬绿色染料检测的自噬液泡积累和自噬流。Hela细胞用以下试剂进行模拟诱导，0.2%DMSO（A）和100uM Clonidine hydrochloride（B）进行诱导，5uM Loperamide hydrochloride和1μM PP242 hydrate（D）在37℃
诱导12小时。处理后，细胞在37℃下用CVTO-ID0Gr een Detection reagent解育10分钟，再用分析缓中液清洗。细胞核被Hoechst 33342染料复染为蓝色。
避免非特异性溶酶体染色的背景干扰
相比其他MDC染色溶酶体检测，CYTO-ID@绿色染料无溶酶体染色背景，CYTO-ID@Autophagy kit无需紫外活细胞分析，在显微镜共标记应用中，与Hoechst染料兼容。
使用流式细胞仪通过CVTO-ID@Autophagy Detection Kit分析自噬
对照组（红线峰值）无诱导，10uM Tamoxifen（ALX-550-095）处理Jurkat细胞（白血病T细胞）（蓝线峰值）。处理18小时后，细胞结合CYTO-ID@绿色检测试剂，然后不经过流式细胞仪清洗，进行分析。结果通过直方图呈现。对照组细胞被染色，但荧光亮度低。实验组中，CVTO-ID@绿色荧光信号增加约2倍，表明Tamoxifen处理能引起Jurkat细胞中自噬的增加。
使用mTOR激酶抑制剂Rapamycin处理HepG2细胞孵育过夜，结果显示CYTO-ID@染色信号升高。
CYTO-ID@绿色染料大多与RFP-LC3蛋白共定位。0.1uM Rapamycin（典型自噬诱导剂）处理过夜，转染Hela细胞表达RFP-LC3。A:CYTO-ID@Green染色；B:RFP-LC3；C：合成图像。
Product Specification
| Applications: | Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS
|
| Application Notes: | The CYTO-ID® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy, flow cytometry, and microplate reader. |
| Quality Control: | A sample from each lot of CYTO-ID® Autophagy detection kit is used to stain Jurkat cells and analyzed by flow cytometry, using the procedures described in the user manual. The MAF values for the samples were greater than 25. The percentage of viable cells in the control samples is > 90% and > 80% in the treated samples. |
| Quantity: | For -K200 size:
200 flow cytometry assays, 250 microscopy assays or 3 x 96-well microplate assays.
For -0050 size:
50 flow cytometry assays, 60 microscopy assays or 1 x 96-well microplate assays. |
| Use/Stability: | With proper storage, the kit components are stable for one year from date of receipt. |
| Handling: | Protect from light. Avoid freeze/thaw cycles. |
| Shipping: | Shipped on Blue Ice |
| Short Term Storage: | -20°C |
| Long Term Storage: | -80°C |
| Contents: | CYTO-ID® Green Detection Reagent
Hoechst 33342 Nuclear Stain
Autophagy Inducer (Rapamycin)
Chloroquine Control
10X Assay Buffer |
| Scientific Background: | Autophagy is a stress-induced protective mechanism. Less active under basal conditions, the mechanism is utilized by eukaryotic cells through lysosome-mediated bulk degradation of cellular contents when subjected to certain hostile conditions such as nutrient depletion and chemical or environmental stress. The role of increased autophagic activity in the pathology of cancer, neurodegeneration, cardiovascular disease and diabetes has become widely recognized and commonly studied. Induction of autophagic flux can be visualized by enhanced accumulation of autophagic vesicles if lysosomal function is inhibited, preventing removal of these vesicles. |
| Technical Info/Product Notes: | The CYTO-ID® Autophagy Detection kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications.
Featured in:
Nature Methods - Autophagy: eat thyself, sustain thyself - Nature Methods, 12.2015
Genetic Engineering & Biotechnology News - HTS Profiling Method for Autophagy-Modulators
Application Notes:
Autophagy Analysis Using Object Spot Counting Using Gen5 to Analyze the Size and Number of Autophagosomes Per Nuclei
Towards Understanding the Molecular Basis of Parkinson’s Disease: Cell-based Model of Mitophagy and Aggresome Accumulation
Response Profiles of Known Autophagy-Modulators Across Multiple Cell Lines: Using CYTO-ID® Autophagy Dye to assess Compound Activity and Toxicity
Cell-Based Screening of Focused Bioactive Compound Libraries: Assessing Small Molecule Modulators of the Canonical Wnt Signaling and Autophagy-Lysosome Pathways
A Novel Image-Based Cytometry Method for Autophagy Detection in Living Cells
Predictive High-Content/High-Throughput Assays for Hepatotoxicity Using Induced Pluripotent Stem Cell (iPSC)-Derived Hepatocytes
Visualizing subcellular vesicles to quantitate autophagy in neuronal cells
Cited samples:
For an overview on cited samples please click here. |
| Protocol: | A detailed protocol for FC in primary BMDCs can be found on bioprotocol.org:
Flow Cytometric Analysis of Autophagic Activity with CYTO-ID Staining in Primary Cells by M. Stankov, et al. |
Product Literature References
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Full Text Apoptosis, Paraptosis and Autophagy: Death and Survival Pathways Associated with Photodynamic Therapy: D. Kessel; Photochem. Photobiol.
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Are There Thresholds in Glioblastoma Cell Death Responses Triggered by Temozolomide?: Y. He & B. Kaina; Int. J. Mol. Sci.
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Full Text Bromelain inhibits the ability of colorectal cancer cells to proliferate via activation of ROS production and autophagy: T.C. Chang, et al.; PLoS One
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Full Text Disturbances in H+ dynamics during environmental carcinogenesis: D. Lagadic-Gossmann, et al.; Biochimie
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Dopamine-melanin nanoparticles scavenge reactive oxygen and nitrogen species and activate autophagy for osteoarthritis therapy: G. Zhong, et al.; Nanoscale
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Enhanced Autophagy Contributes to Reduced Viral Infection in Black Flying Fox Cells: E.D. Laing, et al.; Viruses
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Application(s): Fluorescence Microscopy of HEK293T cells,
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Full Text Examining Cardiomyocyte Dysfunction Using Acute Chemical Induction of an Ageing Phenotype: S. Masoud, et al.; Int. J. Mol. Sci.
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Full Text Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress: H.K. Sung, et al.; Sci. Rep.
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Full Text Leptin stimulates autophagy/lysosome-related degradation of long-lived proteins in adipocytes: N. Goldstein, et al.; Adipocyte
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Full Text Neural stem cell-derived small extracellular vesicles attenuate apoptosis and neuroinflammation after traumatic spinal cord injury by activating autophagy: Y. Rong, et al.; Cell Death Dis.
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Full Text Pivotal role of mitophagy in response of acute myelogenous leukemia to a ceramide-tamoxifen-containing drug regimen: S.A.F. Morad, et al.; Exp. Cell Res.
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Propyl gallate inhibits hepatocellular carcinoma cell growth through the induction of ROS and the activation of autophagy: P.L. Wei, et al.; PLoS One
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Full Text SPARC induces phenotypic modulation of human brain vascular smooth muscle cells via AMPK/mTOR-mediated autophagy: T. Li, et al.; Neurosci. Lett.
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Targeting autophagy potentiates the anti-tumor effect of PARP inhibitor in pediatric chronic myeloid leukemia: Y. Liu, et al.; AMB Express
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Activation of RIG-I-Mediated Antiviral Signaling Triggers Autophagy Through the MAVS-TRAF6-Beclin-1 Signaling Axis: N.R. Lee, et al.; Front. Immunol.
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Full Text Acute exposure to organic and inorganic sources of copper: Differential response in intestinal cell lines: J. Keenan, et al.; Food Sci. Nutr.
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Full Text Association between autophagy and inflammation in patients with rheumatoid arthritis receiving biologic therapy: Y.M. Chen, et al.; Arthritis Res. Ther.
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Application(s): Flow cytometry to detect autophagosome levels in circulating immune cells from patients with rheumatoid arthritis (RA),
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Full Text cAMP-mediated autophagy inhibits DNA damage-induced death of leukemia cells independent of p53: S. Skah, et al.; Oncotarget
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Full Text Conjugation with Phenylalanine Enhances Autophagy-Inducing Activity of (-)-Epigallocatechin Gallate in Hepatic Cells: Y.M. Lee, et al.; J. Agric. Food Chem.
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Depletion of gamma-glutamylcyclotransferase in cancer cells induces autophagy followed by cellular senescence: L. Taniguchi, et al.; Am. J. Cancer Res.
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Full Text Heme oxygenase-1 induction mediates chemoresistance of breast cancer cells to pharmorubicin by promoting autophagy via PI3K/Akt pathway: L. Pei, et al.; J. Cell. Mol. Med.
22, 5311 (2018),
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HMGB1 knockdown increases MM cell vulnerability by regulating autophagy and DNA damage repair: X. Guo, et al.; J. Exp. Clin. Cancer Res.
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Full Text Hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in A549 cells: Y. Wei, et al.; Oncol. Lett.
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Live Mycobacterium leprae inhibits autophagy and apoptosis of infected macrophages and prevents engulfment of host cell by phagocytes: Y. Ma, et al.; Am. J. Transl. Res.
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Full Text Multiscale and Multimodal Approaches to Study Autophagy in Model Plants: J. Marion, et al.; Cells
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Full Text Naïve CD8+ T-Cells Engage a Versatile Metabolic Program Upon Activation in Humans and Differ Energetically From Memory CD8+ T-Cells: F. Nicoli, et al.; Front. Immunol.
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Full Text Novel small molecule SIRT2 inhibitors induce cell death in leukemic cell lines: T. Kozako, et al.; BMC Cancer
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Full Text Partial Hepatectomy-Induced Upregulation of miR-1907 Accelerates Liver Regeneration by Activation Autophagy: T. Lu, et al.; Biomed. Res. Int.
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Full Text Serotonin induced hepatic steatosis is associated with modulation of autophagy and notch signaling pathway: S. Niture, et al.; Cell Commun. Signal.
16, 78 (2018),
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Full Text Signaling Lymphocyte Activation Molecule Family 5 Enhances Autophagy and Fine-Tunes Cytokine Response in Monocyte-Derived Dendritic Cells via Stabilization of Interferon Regulatory Factor 8: Z. Agod, et al.; Front Immunol.
9, 62 (2018),
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Full Text TP53 is required for BECN1- and ATG5-dependent cell death induced by sphingosine kinase 1 inhibition: S. Lima, et al.; Autophagy
11, 1 (2018),
Abstract;
Adipose tissue conditioned media support macrophage lipid-droplet biogenesis by interfering with autophagic flux: S. Bechor, et al.; Biochim. Biophys. Acta Mol. Cell Biol. Lipids
1862, 1001 (2017),
Application(s): Co-localisation with LC3-mCherry in macrophages,
Abstract;
Akt targeting as a strategy to boost chemotherapy efficacy in non-small cell lung cancer through metabolism suppression: M. Le Grand, et al.; Sci. Rep.
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Full Text Ambroxol enhances anti-cancer effect of microtubule-stabilizing drug to lung carcinoma through blocking autophagic flux in lysosome-dependent way: X. Zhang, et al.; Am. J. Cancer Res.
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Autophagy maintains the metabolism and function of young and old (hematopoietic) stem cells: T.T. Ho, et al.; Nature
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Full Text Chloroquine inhibits human CD4+ T-cell activation by AP-1 signaling modulation: R.L.J. Schmidt, et al.; Sci. Rep.
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Application(s): Flow cytometry of human PBMC-derived, activated CD4+ T-Cells,
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Full Text Inhibition of autophagy as a treatment strategy for p53 wild-type acute myeloid leukemia: H. Folkerts, et al.; Cell Death Dis.
8, e2927 (2017),
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Full Text Novel insights into the antioxidant role of tauroursodeoxycholic acid in experimental models of Parkinson's disease: A.I. Rosa, et al.; Biochim. Biophys. Acta Mol. Basis Dis.
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Polyethylene glycol-functionalized poly (Lactic Acid-co-Glycolic Acid) and graphene oxide nanoparticles induce pro-inflammatory and apoptotic responses in Candida albicans-infected vaginal epithelial cells: R.D. Wagner, et al.; PLoS One
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Abstract;
Full Text Protein kinase C-alpha suppresses autophagy and induces neural tube defects via miR-129-2 in diabetic pregnancy: F. Wang, et al.; Nat. Commun.
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Abstract;
Full Text Salinomycin inhibits cholangiocarcinoma growth by inhibition of autophagicflux: J. Klose, et al.; Oncotarget
9, 3619 (2017),
Application(s): Autophagic flux in Cholangiocarcinoma cells,
Abstract;
Full Text Silica sub-microspheres induce autophagy in an endocytosis dependent manner: D. Huang, et al.; RSC Adv.
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Full Text SPARC paucity alleviates superoxide-mediated oxidative stress, apoptosis, and autophagy in diabetogenic hepatocytes: K.R. Aseer, et al.; Free Radic. Biol. Med.
108, 874 (2017),
Application(s): Fluorescence microplate reader using rat hepatocytes,
Abstract;
Sub-lethal oxidative stress induces lysosome biogenesis via a lysosomal membrane permeabilization-cathepsin-caspase 3-transcription factor EB-dependent pathway: S.M. Leow, et al.; Oncotarget
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Abstract;
Full Text Therapeutic effects of the euglenoid ichthyotoxin, euglenophycin, in colon cancer: A.B. Cabang, et al.; Oncotarget
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Abstract;
Full Text UBE2L6/UBCH8 and ISG15 attenuate autophagy in esophageal cancer cells: C.M. Falvey, et al.; Oncotarget
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Abstract;
Full Text Vorinostat and metformin sensitize EGFR-TKI resistant NSCLC cells via BIM-dependent apoptosis induction: H. Chen, et al.; Oncotarget
8, 93825 (2017),
Abstract;
Full Text A comparison of strategies for immortalizing mouse embryonic fibroblasts: M.M. St. Amad, et al.; J. Biol. Methods
3, e41 (2016),
Application(s): Detected autophagy induction in SV40 transformed versus serially passed MEFs,
Full Text Activation of autophagy by FOXO3 regulates redox homeostasis during osteogenic differentiation: M.C. Gómez-Puerto, et al.; Autophagy
12, 1804 (2016),
Abstract;
Full Text An mtDNA mutation accelerates liver aging by interfering with the ROS response and mitochondrial life cycle: J. Niemann, et al.; Free Radic. Biol. Med.
102, 174 (2016),
Abstract;
Astemizole-Histamine induces Beclin-1-independent autophagy by targeting p53-dependent crosstalk between autophagy and apoptosis: R. Jakhar, et al.; Cancer Lett.
372, 89 (2016),
Application(s): Flow cytometry analysis,
Abstract;
Atg5 Is Essential for the Development and Survival of Innate Lymphocytes: T.E. O'Sullivan, et al.; Cell Rep.
15, 1910 (2016),
Application(s): Liver lymphocytes were harvested and stained with cell surface antibodies and then incubated with 1:400 Cyto-ID Autophagy Detection Reagent,
Abstract;
Full Text Atg7 suppression enhances chemotherapeutic agent sensitivity and overcomes stroma-mediated chemoresistance in acute myeloid leukemia: S. Piya, et al.; Blood
128, 1260 (2016),
Application(s): Flow Cytometry in human leukemic cell lines,
Abstract;
Full Text Autophagy and apoptosis: studies on the effects of bisthiosemicarbazone copper(ii) complexes on p53 and p53-null tumour cell lines: F. Bisceglie et al.; Metallomics
8, 1255 (2016),
Abstract;
Autophagy and Mitochondrial Dysfunction in Tenon Fibroblasts from Exfoliation Glaucoma Patients: A. Want, et al.; PLoS One
11, e0157404 (2016),
Application(s): Comparing autophagic flux rates between the XFS and POAG derived TF lines,
Abstract;
Full Text Autophagy in siRNA-mediated PRKAR1A knockdown canine osteosarcoma cells: J. Wong; (2016), (master's thesis);
Application(s): Fluorescence microscopy to visualize fluorescnt probe tagged proteins to study autophagic activity of cells,
Full Text Autophagy-related gene 5 and Wnt5 signaling pathway requires differentiation of embryonic stem cells into odontoblast-like cells: N. Ozeki, et al.; Exp. Cell Res.
341, 92 (2016),
Application(s): Autophagy flux,
Abstract;
Blockade of Inhibitors of Apoptosis Proteins in Combination with Conventional Chemotherapy Leads to Synergistic Antitumor Activity in Medulloblastoma and Cancer Stem-Like Cells: S.M. Chen, et al.; PLoS One
11, e0161299 (2016),
Application(s): Detection of autophagy, cell lysates,
Abstract;
Full Text Bone morphogenetic protein-induced cell differentiation involves Atg7 and Wnt16 sequentially in human stem cell-derived osteoblastic cells: N. Ozeki, et al.; Exp. Cell. Res.
4827, 30181 (2016),
Application(s): Autophagy flux by immunofluorescence microscopy,
Abstract;
Cationic liposomes induce cell necrosis through lysosomal dysfunction and late-stage autophagic flux inhibition: K. Yang, et al.; Nanomedicine (Lond.)
11, 3117 (2016),
Abstract;
DHA-induced stress response in human colon cancer cells-focus on oxidative stress and autophagy: K. Pettersen, et al.; Free Radic. Biol. Med.
90, 158 (2016),
Application(s): Autophagy determined by flow cytometry,
Abstract;
Diosgenin induces ROS-dependent autophagy and cytotoxicity via mTOR signaling pathway in chronic myeloid leukemia cells: S. Jiang, et al.; Phytomedicine
23, 243 (2016),
Application(s): Confocal immunofluorescence,
Abstract;
Downregulated endogenous sulfur dioxide/aspartate aminotransferase pathway is involved in angiotensin II-stimulated cardiomyocyte autophagy and myocardial hypertrophy in mice: Q. Chen, et al.; Int. J. Cardiol.
225, 392 (2016),
Application(s): Examine the effect of endogenous SO2 on autophagy in Ang II-stimulated cardiomyocytes,
Abstract;
Efavirenz causes oxidative stress, endoplasmic reticulum stress, and autophagy in endothelial cells: M. Weiss, et al.; Cardiovasc. Toxicol.
16, 90 (2016),
Abstract;
IRS1 deficiency protects β-cells against ER stress-induced apoptosis by modulating sXBP-1 stability and protein translation: T. Takatani, et al.; Sci. Rep.
6, 28177 (2016),
Application(s): Autophagy assay, to examine the effects of eEF2K activity on autophagy,
Abstract;
Full Text Lithocholic acid induces endoplasmic reticulum stress, autophagy and mitochondrial dysfunction in human prostate cancer cells: A.A. Gafar, et al.; PeerJ
4, e2445 (2016),
Abstract;
Full Text MiR-193b promotes autophagy and non-apoptotic cell death in oesophageal cancer cells: M.J. Nyhan, et al.; BMC Cancer
16, 101 (2016),
Application(s): Assay used to stain live cells,
Abstract;
Full Text Trifloxystrobin-induced mitophagy through mitochondrial damage in human skin keratinocytes: Y. Jang, et al.; J. Toxicol. Sci.
41, 731 (2016),
Abstract;
(Pro)renin receptor regulates autophagy and apoptosis in podocytes exposed to high glucose: C. Li, et al.; Am. J. Physiol. Endocrinol. Metab.
309, E302 (2015),
Application(s): Confocal microscopy using mouse podocytes,
Abstract;
A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications: S. Guo, et al.; Autophagy
11, 560 (2015),
Application(s): Fluorescent detection, Microplate Reader ,
Abstract;
Full Text A Systems Approach Identifies Essential FOXO3 Functions at Key Steps of Terminal Erythropoiesis: R. Liang, et al.; PLoS Genet.
11, e1005526 (2015),
Application(s): Autophagy flux measured by flow cytometry ,
Abstract;
Full Text ABT-888 enhances cytotoxic effects of temozolomide independent of MGMT status in serum free cultured glioma cells: R.K. Balvers, et al.; J. Transl. Med.
13, 74 (2015),
Application(s): Assay,
Abstract;
Full Text Activation of autophagy in response to nanosecond pulsed electric field exposure: J.C. Ullery, et al.; Biochem. Biophys. Res. Commun.
458, 411 (2015),
Application(s): Fluorescence microscopy using U937 monocyte and CHO-K1 cell lines,
Abstract;
Aflatoxin biosynthesis is a novel source of reactive oxygen species-a potential redox signal to initiate resistance to oxidative stress?: L.V. Roze, et al.; Toxins (Basel).
7, 1411 (2015),
Application(s): Assay,
Abstract;
Full Text Alisertib induces cell cycle arrest and autophagy and suppresses epithelial-to-mesenchymal transition involving PI3K/Akt/mTOR and sirtuin 1-mediated signaling pathways in human pancreatic cancer cells: F. Wang, et al.; Drug Des. Devel. Ther.
9, 575 (2015),
Application(s): Flow cytometry using PANC-1 and BxPC-3 pancreatic cancer cell lines,
Abstract;
Full Text Alisertib, an Aurora kinase A inhibitor, induces apoptosis and autophagy but inhibits epithelial to mesenchymal transition in human epithelial ovarian cancer: Y.H. Ding, et al.; Drug Des. Devel. Ther.
9, 425 (2015),
Application(s): Flow cytometry using SKOV3 and OVCAR-4 ovarian cancer cell lines,
Abstract;
Full Text Alisertib, an Aurora kinase A inhibitor, induces apoptosis and autophagy but inhibits epithelial to mesenchymal transition in human epithelial ovarian cancer cells: Y.H. Ding, et al.; Drug Des. Devel. Ther.
9, 425 (2015),
Abstract;
Full Text Andrographolide Analogue Induces Apoptosis and Autophagy Mediated Cell Death in U937 Cells by Inhibition of PI3K/Akt/mTOR Pathway: D. Kumar, et al.; PLoS One
10, e0139657 (2015),
Application(s): Flow cytometric analysis of Cyto-ID Green Detection Reagent ,
Abstract;
Full Text Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells: F. Lang, et al.; PLoS One
10, e0129196 (2015),
Application(s): Live Cell Imaging,
Abstract;
Full Text Araguspongine C induces autophagic death in breast cancer cells through suppression of c-Met and HER2 receptor tyrosine kinase signaling: M.R. Akl, et al.; Mar. Drugs
13, 288 (2015),
Application(s): Flow cytometry using BT-474 breast cancer cell line,
Abstract;
Full Text Autocrine VEGF maintains endothelial survival through regulation of metabolism and autophagy: C.K. Domigan, et al.; J. Cell. Sci.
128, 2236 (2015),
Abstract;
Autophagy is activated in systemic lupus erythematosus and required for plasmablast development: A.J. Clarke, et al.; Ann. Rheum. Dis.
74, 912 (2015),
Abstract;
Full Text Autophagy limits proliferation and glycolytic metabolism in acute myeloid leukemia: A.S. Watson, et al.; Cell Death Discov.
1, 15008 (2015),
Application(s): CytoID assay in human and mouse HSCs,
Abstract;
Full Text Baicalin inhibits autophagy induced by influenza A virus H3N2: H.Y. Zhu, et al.; Antiviral Res.
113, 62 (2015),
Application(s): Fluorescence microscopy using A549 human lung cancer cell line,
Abstract;
Bardoxolone methyl induces apoptosis and autophagy and inhibits epithelial-to-mesenchymal transition and stemness in esophageal squamous cancer cells: Y.Y. Wang, et al.; Drug Des. Devel. Ther.
9, 993 (2015),
Application(s): Flow Cytometry,
Abstract;
Full Text Cell-penetrating peptide derived from human eosinophil cationic protein inhibits mite allergen Der p 2 induced inflammasome activation: S.J. Yu, et al.; PLoS One
10, e0121393 (2015),
Application(s): Flow cytometry of THP-1 leukemia cell line,
Abstract;
Full Text Chemoproteomics Reveals Novel Protein and Lipid Kinase Targets of Clinical CDK4/6 Inhibitors in Lung Cancer: N.J. Sumi, et al.; ACS Chem. Biol.
10, 2680 (2015),
Application(s): Quantification of autophagosomes,
Abstract;
Circulating hemocytes from larvae of the Japanese rhinoceros beetle Allomyrina dichotoma (Linnaeus) (Coleoptera: Scarabaeidae) and the cellular immune response to microorganisms: S. Hwang, et al.; PLoS One
10, e0128519 (2015),
Application(s): Fluorescence microscopy using hemocytes from Japanese rhinoceros beetle Allomyrina dichotoma larvae,
Abstract;
Full Text Citreoviridin induces ROS-dependent autophagic cell death in human liver HepG2 cells: Y.N. Liu, et al.; Toxicon.
95, 30 (2015),
Application(s): Fluorescence microscopy using HepG2 cell line,
Abstract;
Clozapine induces autophagic cell death in non-small cell lung cancer cells: Y.C. Yin, et al.; Cell. Physiol. Biochem.
35, 945 (2015),
Abstract;
Coffee and caffeine potentiate the antiamyloidogenic activity of melatonin via inhibition of Aβ oligomerization and modulation of the Tau-mediated pathway in N2a/APP cells: L.F. Zhang, et al.; Drug Des. Devel. Ther.
9, 241 (2015),
Application(s): Flow Cytometry,
Abstract;
Full Text Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture: H. Burckel, et al.; Oncol. Rep.
33, 471 (2015),
Abstract;
Danusertib Induces Apoptosis, Cell Cycle Arrest, and Autophagy but Inhibits Epithelial to Mesenchymal Transition Involving PI3K/Akt/mTOR Signaling Pathway in Human Ovarian Cancer Cells: D. Zi, et al.; Int. J. Mol. Sci.
16, 27228 (2015),
Application(s): Confocal fluorescence microscopy,
Abstract;
Full Text Danusertib, a potent pan-Aurora kinase and ABL kinase inhibitor, induces cell cycle arrest and programmed cell death and inhibits epithelial to mesenchymal transition involving the PI3K/Akt/mTOR-mediated signaling pathway in human gastric cancer AGS and NCI-N78 cells: C.X. Yuan, et al.; Drug Des. Devel. Ther.
9, 1293 (2015),
Application(s): Flow cytometry using AGS and NCI-N78 gastric cancer cell lines,
Abstract;
Full Text Defective autophagy in vascular smooth muscle cells alters contractility and Ca²⁺ homeostasis in mice: C.F. Michiels, et al.; Am. J. Physiol. Heart Circ. Physiol.
308, H557 (2015),
Abstract;
Effects of cyclodextrins on GM1‐gangliosides in fibroblasts from GM1‐gangliosidosis patients: Y. Maeda, et al.; J. Pharm. Pharmacol.
67, 1133 (2015),
Abstract;
Endurance exercise training induces fat depot-specific differences in basal autophagic activity: G. Tanaka, et al.; Biochem. Biophys. Res. Commun.
466, 512 (2015),
Application(s): Detect the formation of autophagosomes,
Abstract;
Erbin is a novel substrate of the Sag-βTrCP E3 ligase that regulates KrasG12D-induced skin tumorigenesis: C.M. Xie, et al.; J. Cell. Biol.
209, 721 (2015),
Abstract;
Evaluation of Antitumor Effects of Folate-Conjugated Methyl-β-cyclodextrin in Melanoma: K. Motoyama, et al.; Biol. Pharm. Bull.
38, 374 (2015),
Application(s): Fluorescence Microscopy,
Abstract;
Full Text Exchange protein directly activated by cAMP 1 promotes autophagy during cardiomyocyte hypertrophy: A.C. Laurent, et al.; Cardiovasc. Res.
105, 55 (2015),
Application(s): Fluorescence microscopy using rat neonatal ventricular myocytes,
Abstract;
Glutathione-S-transferase omega 1 (GSTO1-1) acts as mediator of signaling pathways involved in aflatoxin B1-induced apoptosis-autophagy crosstalk in macrophages: S. Paul, et al.; Free Radic. Biol. Med.
89, 1218 (2015),
Application(s): Determination of autophagy with immunocytochemistry ,
Abstract;
GMI, an immunomodulatory protein from Ganoderma microsporum, potentiates cisplatin-induced apoptosis via autophagy in lung cancer cells: I.L. Hsin, et al.; Mol. Pharm.
12, 1534 (2015),
Abstract;
Induction of apoptosis and autophagy via sirtuin1- and PI3K/Akt/mTOR-mediated pathways by plumbagin in human prostate cancer cells: Z.W. Zhou, et al.; Drug Des. Devel. Ther.
9, 1511 (2015),
Application(s): Assay,
Abstract;
Full Text Induction of autophagy is a key component of all-trans-retinoic acid-induced differentiation in leukemia cells and a potential target for pharmacologic modulation: N. Orfali, et al.; Exp. Hematol.
43, 781 (2015),
Application(s): Flow cytometry analysis of NB4 and HL60 promyelocytic leukemia cell lines,
Abstract;
Inhibition of Autophagy Potentiated the Antitumor Effect of Nedaplatin in Cisplatin-Resistant Nasopharyngeal Carcinoma Cells: Z. Liu, et al. ; PLoS One
10, e0135236 (2015),
Application(s): Cell culture,
Abstract;
Full Text Inhibition of mitotic Aurora kinase A by alisertib induces apoptosis and autophagy of human gastric cancer AGS and NCI-N78 cells: C.X. Yuan, et al.; Drug Des. Devel. Ther.
9, 487 (2015),
Application(s): Flow cytometry using AGS and NCI-N78 gastric cancer cell lines,
Abstract;
Full Text Interferon Regulatory Factor-1 signaling regulates the switch between autophagy and apoptosis to determine breast cancer cell fate: J.L. Schwartz-Roberts, et al.; Cancer Res.
75, 1046 (2015),
Abstract;
Interplay of Oxidative Stress and Autophagy in PAMAM Dendrimers-Induced Neuronal Cell Death : Y. Li, et al.; Theranostics
5, 1363 (2015),
Application(s): Confocal fluorescence assay,
Abstract;
Full Text Invariant NKT cells require autophagy to coordinate proliferation and survival signals during differentiation: B. Pei, et al.; J. Immunol.
194, 5872 (2015),
Abstract;
Involvement of fish signal transducer and activator of transcription 3 (STAT3) in nodavirus infection induced cell death: Y. Huang, et al.; Fish Shellfish Immunol.
43, 241 (2015),
Application(s): Fluorescence microscopy of Grouper (fish) brain cells,
Abstract;
Is the autophagy a friend or foe in the silver nanoparticles associated radiotherapy for glioma?: H. Wu, et al.; Biomaterials
62, 47 (2015),
Application(s): Fluorescence microscopy using U251 human glioma cell line,
Abstract;
Kaposi's sarcoma-associated herpesvirus induces Nrf2 activation in latently infected endothelial cells through SQSTM1 phosphorylation and interaction with polyubiquitinated Keap1: O. Gjyshi, et al.; J. Virol.
89, 2268 (2015),
Abstract;
KLF4-SQSTM1/p62-associated prosurvival autophagy contributes to carfilzomib resistance in multiple myeloma models: I. Riz, et al.; Oncotarget
6, 17814 (2015),
Application(s): FACS,
Abstract;
Full Text Lithium modulates autophagy in esophageal and colorectal cancer cells and enhances the efficacy of therapeutic agents in vitro and in vivo: T.R. O'Donovan, et al.; PLoS One
10, e0134676 (2015),
Application(s): Flow cytometry analysis using human esophageal and murine colon cancer cell lines,
Abstract;
Full Text Methicillin-Resistant Staphylococcus aureus Adaptation to Human Keratinocytes: G. Soong, et al.; MBio.
6, e00289-15 (2015),
Application(s): Assay,
Abstract;
Full Text Mevalonate pathway regulates cell size homeostasis and proteostasis through autophagy: T.P. Miettinen, et al.; Cell Rep.
13, 2610 (2015),
Application(s): Flow cytometry analysis of autophagy using Jurkat, U2OS, Kc167 and HUVEC cells,
Abstract;
MiR-29b replacement inhibits proteasomes and disrupts aggresome+autophagosome formation to enhance the antimyeloma benefit of bortezomib: S. Jagannathan, et al.; Leukemia
29, 727 (2015),
Application(s): Detection of autophagy by fluorescence microscopy in multiple myeloma cell lines,
Abstract;
Full Text Molecular chaperone GRP78 enhances aggresome delivery to autophagosomes to promote drug resistance in multiple myeloma: M.A. Abdel Malek, et al.; Oncotarget
6, 3098 (2015),
Application(s): Confocal Microscopy,
Abstract;
Full Text Molecular cloning and characterization of autophagy-related gene TmATG8 in Listeria-invaded hemocytes of Tenebrio molitor: H. Tindwa, et al.; Dev. Comp. Immunol.
51, 88 (2015),
Application(s): Fluorescence microscopy using hemocytes from Tenebrio molitor larvae,
Abstract;
Molecular pathway of near-infrared laser phototoxicity involves ATF-4 orchestrated ER stress: I. Khan, et al.; Sci. Rep.
5, 10581 (2015),
Application(s): Fluorescence microscopy autophagy assay,
Abstract;
Full Text N-Myc and STAT Interactor regulates autophagy and chemosensitivity in breast cancer cells: B.J. Metge, et al.; Sci. Rep.
5, 11995 (2015),
Application(s): Fluorescent detection,
Abstract;
Full Text Novel autophagy inducers lentztrehaloses A, B and C: S.I. Wada, et al.; J. Antibiot. (Tokyo)
68, 521 (2015),
Application(s): Fluorescence microscopy using Mewo melanoma and OVK18 ovarian cancer cell lines,
Abstract;
Novel small-molecule SIRT1 inhibitors induce cell death in adult T-cell leukaemia cells: T. Kozako, et al.; Sci. Rep.
5, 11345 (2015),
Application(s): Flow cytometry using a variety of cancer cell lines,
Abstract;
Full Text Novel targeting of PEGylated liposomes for codelivery of TGF-β1 siRNA and four antitubercular drugs to human macrophages for the treatment of mycobacterial infection: a quantitative proteomic study: N. Niu, et al. ; Drug Des. Devel. Ther.
9, 4441 (2015),
Application(s): Autophagy of human macrophages by flow cytometry,
Abstract;
Paraptosis cell death induction by the thiamine analog benfotiamine in leukemia cells: N. Sugimori, et al.; PLoS One
10, e0120709 (2015),
Application(s): Flow cytometry using HL60 leukemia cell line,
Abstract;
Full Text Plumbagin induces G2/M arrest, apoptosis, and autophagy via p38 MAPK- and PI3K/Akt/mTOR-mediated pathways in human tongue squamous cell carcinoma cells: S.T. Pan, et al.; Drug Des. Devel. Ther.
9, 1601 (2015),
Application(s): Assay,
Abstract;
Full Text Pro-apoptotic and pro-autophagic effects of the Aurora kinase A inhibitor alisertib (MLN8237) on human osteosarcoma U-2 OS and MG-63 cells through the activation of mitochondria-mediated pathway and inhibition of p38 MAPK/PI3K/Akt/mTOR signaling pathway: N.K. Niu, et al.; Drug Des. Devel. Ther.
9, 1555 (2015),
Application(s): Assay,
Abstract;
Full Text Reduced FoxO3a expression causes low autophagy in idiopathic pulmonary fibrosis fibroblasts on collagen matrix: J. Im, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol.
309, L552 (2015),
Abstract;
Runx1 Deficiency Decreases Ribosome Biogenesis and Confers Stress Resistance to Hematopoietic Stem and Progenitor Cells: X. Cai, et al.; Cell Stem Cell
17, 165 (2015),
Application(s): Flow Cytometry of mouse hematomoietic stem and progenitor cells (HSPCs),
Abstract;
Full Text S-Adenosyl-L-methionine-competitive inhibitors of the histone methyltransferase EZH2 induce autophagy and enhance drug sensitivity in cancer cells: T.P. Liu, et al.; Anticancer Drugs
26, 139 (2015),
Application(s): Fluorescence microscopy using MDA-MB-231 breast cancer cell line,
Abstract;
Full Text Schisandrin B inhibits cell growth and induces cellular apoptosis and autophagy in mouse hepatocytes and macrophages: implications for its hepatotoxicity: Y. Zhang, et al.; Drug Des. Devel. Ther.
9, 2001 (2015),
Application(s): Flow cytometry using AML-12 hepatocyte and RAW 264.7 leukemia cell lines,
Abstract;
Full Text Src/STAT3-dependent heme oxygenase-1 induction mediates chemoresistance of breast cancer cells to doxorubicin by promoting autophagy: Q. Tan, et al.; Cancer Sci.
106, 1023 (2015),
Abstract;
The CCL2 chemokine is a negative regulator of autophagy and necrosis in luminal B breast cancer cells: W.B. Fang, et al.; Breast Cancer Res. Treat.
150, 309 (2015),
Abstract;
The investigational Aurora kinase A inhibitor alisertib (MLN8237) induces cell cycle G2/M arrest, apoptosis, and autophagy via p38 MAPK and Akt/mTOR signaling pathways in human breast cancer cells: J.P. Li, et al.; Drug Des. Devel. Ther.
9, 1627 (2015),
Application(s): Assay,
Abstract;
Full Text The pan-inhibitor of Aurora kinases danusertib induces apoptosis and autophagy and suppresses epithelial-to-mesenchymal transition in human breast cancer cells: J.P. Li, et al.; Drug Des. Devel. Ther.
9, 1027 (2015),
Application(s): Assay,
Abstract;
Full Text The role of autophagy in the cytotoxicity induced by recombinant human arginase in laryngeal squamous cell carcinoma: C. Lin, et al.; Appl. Microbiol. Biotechnol.
99, 8487 (2015),
Abstract;
A Novel CXCR3-B Chemokine Receptor-induced Growth-inhibitory Signal in Cancer Cells Is Mediated through the Regulation of Bach-1 Protein and Nrf2 Protein Nuclear Translocation : M. Balan & S. Pal; J. Biol. Chem.
289, 3126 (2014),
Application(s): Monitor autophagy in MCF-7 and T47D breast cancer cells by flow cytometry and fluorescence microscopy,
Abstract;
Adaptive responses to glucose restriction enhance cell survival, antioxidant capability, and autophagy of the protozoan parasite Trichomonas vaginalis: K.Y. Huang, et al.; Biochim. Biophys. Acta.
1840, 53 (2014),
Abstract;
Autophagy in the brain of neonates following hypoxia-ischemia shows sex-and region-specific effects: S.N. Weis, et al.; Neuroscience
256, 201 (2014),
Abstract;
Cannabinoid-induced autophagy regulates suppressor of cytokine signaling-3 in intestinal epithelium: L.C. Koay, et al.; Am. J. Physiol. Gastrointest. Liver Physiol.
307, G140 (2014),
Application(s): Detection of autophagy in human colonic epithelial cell line Caco-2 by Confocal imaging,
Abstract;
Full Text Caveolin-1 Is a Critical Determinant of Autophagy, Metabolic Switching, and Oxidative Stress in Vascular Endothelium: T. Shiroto, et al.; PLoS One
9, e87871 (2014),
Abstract;
Full Text Connective tissue diseases: How do autoreactive B cells survive in SLE-autophagy?: N.J. Bernard; Nat. Rev. Rheumatol.
10, 128 (2014), (Review),
Abstract;
Defective Autophagosome Trafficking Contributes to Impaired Autophagic Flux in Coronary Arterial Myocytes Lacking CD38 Gene: Y. Zhang, et al.; Cardiovasc. Res.
102, 68 (2014),
Abstract;
Defects in mitochondrial clearance predispose human monocytes to interleukin-1β hyper-secretion: R. van der Burgh, et al.; J. Biol. Chem.
289, 5000 (2014),
Abstract;
Full Text Early biomarkers of response to carfilzomib in multiple myeloma (MM): Modulation of CXCR4 and induction of autophagy: M. Bhutani, et al.; J. Clin. Oncol. 32, e19572 (2014), Application(s): Quantification of autophagy in malignant plasma cells from bone marrow aspirates by flow cytometry with the Cyto-ID autophagy detection kit,
Enhancement of dynein-mediated autophagosome trafficking and autophagy maturation by ROS in mouse coronary arterial myocytes: M. Xu, et al.; J. Cell. Mol. Med.
18, 2165 (2014),
Abstract;
Full Text Flow Cytometric Analysis of Autophagic Activity with Cyto-ID Staining in Primary Cells: M. Stankov, et al.; Bio-Protocol (2014),
Application(s): FC in primary BMDCs,
Full Text High-Content Assays for Hepatotoxicity Using Induced Pluripotent Stem Cell-Derived Cells: O. Sirenko, et al.; Assay Drug Dev. Technol.
12, 43 (2014),
Abstract;
Full Text Histone deacetylase inhibitors induce apoptosis in myeloid leukemia by suppressing autophagy: M.V. Stankov, et al.; Leukemia
28, 577 (2014),
Abstract;
Histone deacetylase inhibitors potentiate VSV oncolysis in prostate cancer cells by modulating NF-κB dependent autophagy: L. Shulak, et al.; J. Virol.
88, 2927 (2014),
Abstract;
In vitro and in vivo characterization of porcine acellular dermal matrix for gingival augmentation procedures: A.M. Pabst, et al.; J. Periodontal. Res.
49, 371 (2014),
Abstract;
Inhibition of Autophagic Flux by Salinomycin Results in Anti-Cancer Effect in Hepatocellular Carcinoma Cells: J. Klose, et al.; PLoS One
9, e95970 (2014),
Application(s): Autophagy detection in human hepatocellular carcinoma ,
Abstract;
Full Text Inhibition of stress induced premature senescence in presenilin-1 mutated cells with water soluble Coenzyme Q10: D. Ma, et al.; Mitochondrion
17C, 106 (2014),
Application(s): Autophagic vacuoles in Alzheimer's Disease fibroblasts detected with CytoID® Green Autophagy Detection kit,
Abstract;
Involvement of autophagy in recombinant human arginase-induced cell apoptosis and growth inhibition of malignant melanoma cells: Z. Wang, et al.; Appl. Microbiol. Biotechnol.
98, 2485 (2014),
Abstract;
MiR-216a: a link between endothelial dysfunction and autophagy: R. Menghini, et al.; Cell Death Dis.
5, e1029 (2014),
Abstract;
Novel estradiol analogue induces apoptosis and autophagy in esophageal carcinoma cells: E. Wolmarans, et al.; Cell. Mol. Biol. Lett.
19 , 98 (2014),
Application(s): Autophagy detection in esophageal carcinoma SNO cell ,
Abstract;
Novel sorafenib-based structural analogues: in-vitro anticancer evaluation of t-MTUCB and t-AUCMB: A.T. Wecksler, et al.; Anticancer Drugs
25, 433 (2014),
Abstract;
Photodynamic therapy with the novel photosensitizer chlorophyllin f induces apoptosis and autophagy in human bladder cancer cells: D. Lihuan, et al.; Lasers Surg. Med.
46, 319 (2014),
Abstract;
Plumbagin induces apoptotic and autophagic cell death through inhibition of the PI3K/Akt/mTOR pathway in human non-small cell lung cancer cells: Y.C.Li, et al.; Cancer Lett.
344, 239 (2014),
Abstract;
Potential of adenovirus-mediated REIC/Dkk-3 gene therapy for use in the treatment of pancreatic cancer: D. Uchida, et al.; J. Gastroenterol. Hepatol.
29, 973 (2014),
Abstract;
Sirt1 modulates endoplasmic reticulum stress-induced autophagy in heart: A. Guilbert, et al.; Cardiovasc. Res.
103 (suppl 1), S13 (2014),
Application(s): Evaluation of Autophagy in H9c2 cells, rat cardiomyoblasts by flow cytometry,
Full Text STAT3 down regulates LC3 to inhibit autophagy and pancreatic cancer cell growth: J. Gong, et al.; Oncotarget
5, 2529 (2014),
Application(s): Autophagic vacuole formation was detected by microscopy and autophagosome formation was determined by flow cytometry in human pancreatic cancer cells Capan-2,
Abstract;
Full Text T-Cell Autophagy Deficiency Increases Mortality and Suppresses Immune Responses after Sepsis: C.W. Lin, et al.; PLoS One
9, e102066 (2014),
Application(s): Quantification of autophagosomes and autolysosomes staining in CD4+ and CD8+ cell population by flow cytometry ,
Abstract;
Full Text Tetracyclines cause cell stress-dependent ATF4 activation and mTOR inhibition: A. Brüning, et al.; Exp. Cell Res.
320, 281 (2014),
Abstract;
The core autophagy protein ATG4B is a potential biomarker and therapeutic target in CML stem/progenitor cells: K. Rothe, et al.; Blood
123, 3622 (2014),
Application(s): Monitor autophagy flux in hematopoietic stem/progenitor cells,
Abstract;
Androgen deprivation and androgen receptor competition by bicalutamide induce autophagy of hormone-resistant prostate cancer cells and confer resistance to apoptosis: B. Boutin, et al.; Prostate
73, 1090 (2013),
Application(s): Measurement of autophagic flux in prostate cancer cells,
Abstract;
Arenobufagin, a natural bufadienolide from toad vonem, induces apoptosis and autophagy in human hepatocellular carcinoma cells through inhibition of PI3K/Akt/mTOR pathway: D.M. Zhang, et al.; Carcinogenesis
34, 1331 (2013),
Application(s): Autophagy detection in hepatocellular carcinoma,
Abstract;
Autophagy Plays a Critical Role in ChLym-1-Induced Cytotoxicity of Non-Hodgkin's Lymphoma Cells: J. Fan, et al.; PLoS One.
8, e72478 (2013),
Abstract;
Full Text BCL-2 inhibitors sensitize therapy-resistant chronic lymphocytic leukemia cells to VSV oncolysis: S. Samuel, et al.; Mol. Ther.
21, 1413 (2013),
Abstract;
Bleomycin exerts ambivalent antitumor immune effect by triggering both immunogenic cell death and proliferation of regulatory T cells: H. Bugaut, et al.; PLoS One
8, e65181 (2013),
Application(s): Measurement of autophagy by flow cytometry and fluorescence microscopy,
Abstract;
Full Text Celecoxib enhances radiosensitivity of hypoxic glioblastoma cells through endoplasmic reticulum stress: K. Suzuki, et al.; Neuro. Oncol.
15, 1186 (2013),
Abstract;
Chloroquine Engages the Immune System to Eradicate Irradiated Breast Tumors in Mice: J.A. Ratikan, et al.; Int. J. Radiat. Oncol. Biol. Phys.
87, 761 (2013),
Abstract;
Dietary Resveratrol Prevents Development of High-Grade Prostatic Intraepithelial Neoplastic Lesions: Involvement of SIRT1/S6K Axis: G. Li, et al.; Cancer Prev. Res
6, 27 (2013),
Application(s): Effects of Resveratrol on prostate tumorigenesis,
Abstract;
Enhancement of autophagy by simvastatin through inhibition of Rac1-mTOR signaling pathway in coronary arterial myocytes: Y.M. Wei, et al.; Cell. Physiol. Biochem.
31, 925 (2013),
Abstract;
Full Text GX15-070 (obatoclax) induces apoptosis and inhibits cathepsin D and L mediated autophagosomal lysis in antiestrogen resistant breast cancer cells: J.L. Schwartz-Roberts, et al.; Mol. Cancer Ther.
12, 448 (2013),
Application(s): Autophagy detection in breast cancer cells,
Abstract;
Hydroxychloroquine preferentially induces apoptosis of CD45RO+ effector T cells by inhibiting autophagy: A possible mechanism for therapeutic modulation of T cells: J. van Loodregt, et al.; J. Allergy Clin. Immunol.
131, 1443 (2013),
Application(s): Detection of autophagy in CD4+ T cells and PBMC by flow cytometry ,
Abstract;
Full Text Interactions between autophagic and endo-lysosomal markers in endothelial cells: C.L. Oeste, et al.; Histochem. Cell. Biol.
139, 659 (2013),
Abstract;
Involvement of cholesterol depletion from lipid rafts in apoptosis induced by methyl-β-cyclodextrin: R. Onodera, et al.; Int. J. Pharm.
452, 116 (2013),
Application(s): Measurement of autophagy by fluorescence microscopy,
Abstract;
ISG15 deregulates autophagy in genotoxin-treated ataxia telangiectasia cells: S.D. Desai, et al.; J. Biol. Chem.
288, 2388 (2013),
Application(s): Fluorescence microscopy using Ataxia Telangiectasia cells,
Abstract;
Full Text Lysosomal basification and decreased autophagic flux in oxidatively stressed trabecular meshwork cells: Implications for glaucoma pathogenesis: K. Porter, et al.; Autophagy
9, 581 (2013),
Application(s): Autophagy detection by flow cytometry in porcine TM cells,
Abstract;
Full Text Nelfinavir and bortezomib inhibit mTOR activity via ATF4-mediated sestrin-2 regulation: A. Brüning; Mol. Oncol.
7, 1012 (2013),
Abstract;
Recombinant human arginase induced caspase-dependent apoptosis and autophagy in non-Hodgkin's lymphoma cells: X. Zeng, et al.; Cell Death Dis.
4, e840 (2013),
Abstract;
Full Text Regulation of autophagic flux by dynein-mediated autophagosomes trafficking in mouse coronary arterial myocytes: M. Xu, et al.; Biochim. Biophys. Acta.
1833, 3228 (2013),
Abstract;
Renal cancer-selective Englerin A induces multiple mechanisms of cell death and autophagy: R.T. Williams, et al.; J. Exp. Clin. Cancer Res.
32, 57 (2013),
Application(s): Flow cytometry and immunofluorescence of a human kidney carcinoma cell line,
Abstract;
Full Text Saxifragifolin D induces the interplay between apoptosis and autophagy in breast cancer cells through ROS-dependent endoplasmic reticulum stress: J.M. Shi, et al.; Biochem. Pharmacol.
85, 913 (2013),
Application(s): Autophagy detection by flow cytometry in breast cancer cells,
Abstract;
Suppression of autophagy enhanced growth inhibition and apoptosis of interferon-β in human glioma cells: Y. Li, et al.; Mol. Neurobiol.
47, 1000 (2013),
Abstract;
Survival and death strategies in glioma cells: autophagy, senescence and apoptosis triggered by a single type of temozolomide-induced DNA damage: A.V. Knizhnik, et al.; PLoS One
8, e55665 (2013),
Application(s): Autophagy detection by flow cytometry in glioma cells,
Abstract;
Full Text The effect of Zhangfei on the unfolded protein response and growth of cells derived from canine and human osteosarcomas: T. Bergeron, et al.; Vet. Comp. Oncol.
11, 140 (2013),
Application(s): Detection of autophagy in human and canine osteosarcoma,
Abstract;
The mTOR inhibitor RAD001 potentiates autophagic cell death induced by temozolomide in a glioblastoma cell line: E. Josset, et al.; Anticancer Res.
33, 1845 (2013),
Abstract;
Therapeutic Combination of Nanoliposomal Safingol and Nanoliposomal Ceramide for Acute Myeloid Leukemia: T.J. Brown, et al.; J. Leuk.
1, 110 (2013),
Application(s): Detection of autophagy by flow cytometry in Human HL-60 , HL-60/VCR, and murine C1498 cells,
Full Text Type I interferons induce autophagy in certain human cancer cell lines: H. Schmeisser, et al.; Autophagy
9, 683 (2013),
Application(s): Autophagy detection in type I interferon-treated human cancer cell lines,
Abstract;
A novel image-based cytometry method for autophagy detection in living cells: L.L. Chan, et al.; Autophagy
8, 1371 (2012),
Abstract;
Full Text Apoptosis and autophagy have opposite roles on imatinib-induced K562 leukemia cell senescence: C. Drullion, et al.; Cell Death Dis.
3, e373 (2012),
Application(s): Flow cytometry of human CML cells treated with Imatinib,
Abstract;
Full Text Counteracting autophagy overcomes resistance to everolimus in mantle cell lymphoma: L. Rosich, et al.; Clin. Cancer Res.
18, 5278 (2012),
Abstract;
Full Text Heme Oxygenase-1 Promotes Survival of Renal Cancer Cells through Modulation of Apoptosis-and Autophagy-regulating Molecules: P. Banerjee, et al.; J. Biol. Chem.
287, 4962 (2012),
Application(s): Detection of autophagy in human renal cancer cells,
Abstract;
Inhibition of monocarboxylate transporter 2 induces senescence-associated mitochondrial dysfunction and suppresses progression of colorectal malignancies in vivo: I. Lee, et al.; Mol. Cancer Ther.
11, 2342 (2012),
Abstract;
Full Text Mechanism for the induction of cell death in ONS-76 medulloblastoma cells by Zhangfei/CREB-ZF: T.W. Bodnarchuk, et al.; J. Neurooncol.
109, 485 (2012),
Application(s): Detection of autophagy in medulloblastoma cells,
Abstract;
Mitochondrial metabolism in Parkinson's disease impairs quality control autophagy by hampering microtubule-dependent traffic: D.M. Arduíno, et al.; Hum. Mol. Genet.
21, 4680 (2012),
Abstract;
Full Text Proteasome inhibition by quercetin triggers macroautophagy and blocks mTor activity: A.K. Klappan, et al.; Histochem. Cell Biol.
137, 25 (2012),
Abstract;
Reovirus as a viable therapeutic option for the treatment of multiple myeloma: C.M. Thirukkumaran, et al.; Clin. Cancer Res.
18, 4962 (2012),
Application(s): Detection of autophagy in human myeloma cell lines and ex vivo tumor specimens,
Abstract;
Src inhibition with saracatinib reverses fulvestrant resistance in ER-positive ovarian cancer models in vitro and in vivo: F.A. Simpkins, et al.; Clin. Cancer Res.
18, 5911 (2012),
Application(s): Detection of autophagy in human ovarian cancer cells and xenografts,
Abstract;
FoxM1 knockdown sensitizes human cancer cells to proteasome inhibitor-induced apoptosis but not to autophagy: B. Pandit, et al.; Cell Cycle
10, 3269 (2011),
Application(s): Flow cytometry using human cancer cells,
Abstract;
Full Text Monitoring of autophagy in Chinese hamster ovary cells using flow cytometry: J.S. Lee, et al.; Methods
56(3), 375 (2011),
Abstract;
Selective anticancer activity of a hexapeptide with sequence homology to a non-kinase domain of Cyclin Dependent Kinase 4: H.M. Warenius, et al.; Mol. Cancer
10, 72 (2011),
Abstract;
Silibinin triggers apoptotic signaling pathways and autophagic survival response in human colon adenocarcinoma cells and their derived metastatic cells: H. Kauntz, et al.; Apoptosis
16, 1042 (2011),
Abstract;
General Literature References
A live-cell fluorescence microplate assay suitable for monitoring vacuolation arising from drug or toxic agent treatment: J. Coleman, et al.; J. Biomol. Screen.
15, 398 (2010),
Abstract;
Methods in mammalian autophagy research: N. Mizushima, et al.; Cell
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