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品牌：Enzo 货号：ADI-900-057 产品名称：Protein A ELISA kit（蛋白A酶联免疫试剂盒）
ENZO Life Sciences 强势推出的Protein A ELISA kit采用夹心法定量检测单抗制备过程中的Protein A残留，超灵敏，具有极好的批间重复性。
金黄色葡萄球菌细胞壁中的A蛋白（Staphylococal Protein A，SPA）又称Protein A，42kDa，能特异性地结合IgG抗体，通常在大规模抗体制备中用于纯化gG。如果制备的抗体中存在Protein A，会严重降低抗体的质量。
品牌：Enzo 货号：ADI-900-057 产品名称：Protein A ELISA kit（蛋白A酶联免疫试剂盒） ENZO Life Sciences 强势推出的Protein A ELISA kit采用夹心法定量检测单抗制备过程中的Protein A残留，超灵敏，具有极好的批间重复性。 金黄色葡萄球菌细胞壁中的A蛋白（Staphylococal Protein A，SPA）又称Protein A，42kDa，能特异性地结合IgG抗体，通常在大规模抗体制备中用于纯化gG。如果制备的抗体中存在Protein A，会严重降低抗体的质量。
| 品牌 | 货号 | 产品名称 | 规格 |
| Enzo | ADI-900-057 | Protein A ELISA kit 蛋白A酶联免疫试剂盒 | 96 wells |
产品说明书：
ENZO Life Sciences 强势推出的Protein A ELISA kit采用夹心法定量检测单抗制备过程中的Protein A残留，超灵敏，具有极好的批间重复性。
金黄色葡萄球菌细胞壁中的A蛋白（Staphylococal ProteinA，SPA）又称Protein A，42kDa，能特异性地结合IgG抗体，通常在大规模抗体制备中用于纯化1gG。如果制备的抗体中存在Protein A，会严重降低抗体的质量。
·特点
·适用于抗体制备过程中的Protein A残留检测
·可检测任意物种中4种不同结构的天然或重组ProteinA
·超灵敏，检测灵敏度9.01pg/ml，在人IgG中可检测低至1ppm的ProteinA残留
·检测范围：15.63-1000pg/ml
·3h内检测37个样品（复孔），时间短、成本低
A:Natural Protein A from S.aureus（Millipore），n=9；
B:Recombinant Protein A from E.cof（Repligen），n=9；
C:Recombinant Cys-Protein A from E.cof（GE），n=12；D:Recombin ant alkaline-resistant Protein A variant from E.cof（MabSelet SuRe from GE），n=12；途中数据的统一标准差为+/-1。
Product Specification
| Sensitivity: | 9.01 pg/ml (range 15.63 - 1,000 pg/ml) |
| Assay Time: | <3 hours |
| Applications: | ELISA, Colorimetric detection |
| Application Notes: | For the quantitative determination of residual protein A contamination in protein A purified IgG preparations from any species. |
| Species reactivity: | Species independent |
| Shipping: | Blue Ice Not Frozen |
| Long Term Storage: | +4°C |
| Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 13, Wash buffer concentrate,Standard, TMB Substrate, Stop solution 2 |
| Scientific Background: | Staphylococcus aureus Protein A is a 42 kDa cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially the IgG class. The IgG binding domain (domain B) consists of three anti-parallel alpha-helices, the third of which is disrupted when the protein is complexed with Fc. Protein A is commonly used to purify antisera and in commonly used immunodetection and visualization techniques. Protein A participates in a number of protective biological functions including anti-tumor, toxic and carcinogenic activities, thus necessitating the removal of potential Protein A contaminants from antibody preparations for therapeutic use. |
| Technical Info/Product Notes: | Assay recognition of different Protein A constructs from natural Protein A from S. aureus (M), recombinant Protein A from E. coli (R), recombinant Cys-Protein A from E. coli (G), and recombinant alkaline-resistant Protein A variant from E. coli (S) were tested and the resulting concentrations were interpolated from kit standard curve and displayed in the figure provided. Application Notes: Comparison of Assay Methods for the Detection of Residual Protein A in Biological Therapeutics |
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