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上海宇劲生物孙经理 
品牌：AdipoGen 货号：AG-40B-0025-C010、AG-40B-0025-3010
Toll样受体5（TLR5）能识别来自革兰氏阳性菌和革兰氏阴性菌的鞭毛蛋白。受体激活后，会将信号传递到接头蛋白MyD88，TIRAP和TRIF，从而诱导促炎性细胞因子的产生，如TNF-a。鞭毛蛋白是亚基蛋白，可以通过聚合形成细菌鞭毛的细丝。它不仅通过TLR5激活先天免疫系统，还通过细胞内NAIP5/NLRC4（IPAF）炎症小体蛋白激活先天免疫系统。
AdipoGen Life Sciences提供不同类型的低内毒素含量和高纯度的鞭毛蛋白，也包含通路特异性突变体。鞭毛蛋白（NLRC4突变体）（重组）（产品编号AG-40B-0126）仅由TLR5检测，而不是由NLRC4检测，而鞭毛蛋白（TLR5突变体）（重组）（产品编号AG-40B-0127）则仅由NLRC4检测到。
| 品牌 | 货号 | 产品名称 | 规格 |
| Adipogen | AG-40B-0025-C010 | Flagellin (high purity) 鞭毛蛋白（高纯度） | 10 µg |
| Adipogen | Flagellin (high purity) 鞭毛蛋白（高纯度） | 3 x 10 µg |
产品说明书：
AG-40B-0025-Flagellin-high-purity.pdf
| Product Details | |
|---|---|
| Synonyms | FliC |
| Product Type | Protein |
Properties | |
| Source/Host | Salmonella typhimurium strain 14028 |
| Crossreactivity | Human Mouse |
| Specificity | Binds to human and mouse TLR5 (Toll-like receptor 5). |
| Biological Activity | Activation of TLR5 in human epithelial cell assays based on NF-κB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, β-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1µg /mouse. |
| MW | ~54kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data | UniProt link P06179: Flagellin (Salmonella typhimurium) |
Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
·参考文献
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