产品说明书：

AG-25B-0006-anti-Asc-pAb-AL177.pdf

AG_25B_0006_Functional_Assay_Protocol.pdf

·产品详情

别名：Pycard；甲基化诱导沉默的靶点1；TMS1；|Caspase Recruitment Domain-containing Protein 5；细胞凋亡相关Speck样蛋白CARD；CARD5

产品类型：多克隆抗体

·产品属性

克隆号：Cryo-2

免疫原/抗原：对应于N末端人ASc的氨基酸合成肽

来源/宿主：兔

应用：WB:1：1000

    ICC:1：200

    IP：1：200

    IHC:1：500；paraffin sections

   功能实验: 见 Protocol

反应性：人/小鼠

特异性：识别人和小鼠Asc

纯度 ：表位亲和纯化

浓度：1mg/mL

配制：液体，溶解在含10%甘油和0.02%叠氮化钠的PBS缓中液中。

·运输及处理

运输条件：冰袭

短期储存：+4℃

长期储存：-20℃

处理建议：打开后，等分处理并储存于-20℃条件下。避免反复冻融。

使用/稳定性：收货后保存于-20℃条件下，可至少保存1年。

·参考文献

1. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder: L. Agostini, et al.; Immunity 20, 319 (2004)
2. P2X7 Receptor Differentially Couples to Distinct Release Pathways for IL-1beta in Mouse Macrophage: P. Pelegrin, et al.; J. Immunol. 180, 7147 (2008)
3. Inflammatory role of ASC in antigen-induced arthritis is independent of caspase-1, NALP-3, and IPAF: L. Kolly, et al.; J. Immunol. 183, 4003 (2009) (IF)
4. Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinated inflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255 (2012)
5. NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.; Nature 493, 674 (2013) (IF)
6. Measuring inflammasome activation in response to bacterial infection: P. Broz & D.M. Monack; Methods Mol. Biol. 1040, 65 (2013)
7. Promyelocytic leukemia protein interacts with the apoptosis-associated speck-like protein to limit inflammasome activation: J.K. Dowling, et al.; J. Biol. Chem. 289, 6429 (2014)
8. Localization and functionality of the inflammasome in neutrophils: M. Bakele, et al.; J. Biol. Chem. 289, 5320 (2014)
9. The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation: B.S. Franklin, et al.; Nat. Immunol. 15, 727 (2014) (IF)
10. The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014)
11. Aging-associated TNF production primes inflammasome activation and Nlrp3-related metabolic disturbances: F. Bauernfeind, et al.; J. Immunol. 197, 2900 (2016)
12. Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence: M. Beilharz, et al.; Methods Mol. Biol. 1417, 145 (2016)
13. Cell-Free Assay for Inflammasome Activation: Y. Jamilloux & F. Martinon; Methods Mol. Biol. 1417, 207 (2016)
14. The inflammasome pathway in stable COPD and acute exacerbations: R. Faner, et al.; ERJ Open Res. 2, 00002-2016 (2016) (IF)
15. A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly: F.I. Schmidt, et al.; J. Exp. Med. 213, 771 (2016) (IF)
16. Microglia-derived ASC specks cross-seed amyloid-β in Alzheimer's disease: C. Venegas, et al.; Nature 552, 355 (2017) (IF)
17. The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancer: E. Brunetto, et al.; J. Immunother. Cancer 7, 45 (2019) (IF)
18. NLRP3 inflammasome activation drives tau pathology: C. Ising, et al.; Nature 575, 669 (2019) (IF)