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品牌：AdipoGen     货号：AG-20B-0043-C100

中性粒细胞是炎症反应的关键组成部分，因此有助于杀死微生物。另外，最近的证据表明它们参与了免疫应答的发展。 Nimp-R14 mAb可在体内耗尽小鼠中性粒细胞和单核细胞。

名称：抗小鼠中性粒细胞单抗 炎症反应 AG-20B-0043-C100
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品牌货号产品名称规格
AdipoGenAG-20B-0043-C100

anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14)

抗小鼠中性粒细胞单抗 （封闭作用） （Nimp-R14）

100 µg

产品说明书：

AG-20B-0043-anti-Neutrophils-mouse-mAb-blocking-Nimp-R14.pdf


Product Details
Product TypeMonoclonal Antibody


Properties

CloneNimp-R14
IsotypeRat IgG2b
Source/HostPurified from concentrated hybridoma tissue culture supernatant.
Immunogen/AntigenPurified mouse BALB/c neutrophils.
Application

Flow Cytometry:(1:100)
Immunohistochemistry:(1:200; frozen section / paraffin section)
Immunocytochemistry: (1:200)
Functional (Blocking): 250μg / mouse (depletes neutrophils and monocytes in vivo)

CrossreactivityMouse
Specificity

Recognizes mouse neutrophils (surface protein mouse Ly-6G) and may recognize Ly-6Chigh cells (inflammatory monocytes).

Purity≥95% (SDS-PAGE)
Purity DetailProtein G-affinity purified.
Endotoxin Content<0.1EU/μg purified protein (LAL test; Lonza).
Concentration1mg/ml
FormulationLiquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control

Rat IgG2b Isotype Control

Other Product Data

Negative control: AG-35B-0011


Shipping and Handling

ShippingBLUE ICE
Short Term Storage+4°C
Long Term Storage-20°C
Handling AdviceAfter opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/StabilityStable for at least 1 year after receipt when stored at -20°C.


·参考文献

  1. Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies: A. Lopez, et al.;  Brit. J. Haematol. 57, 489 (1984)
  2. Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells: D. McLaren, et al.;  Parasite Immunol. 9, 667 (1987)
  3. Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice: P. Van Lent, et al.; J. Leukoc. Biol. 61, 267 (1997)
  4. Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction: E. Lubberts, et al.; J. Immunol. 163, 4546 (1999)
  5. An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major: F. Tacchini-Cottier, et al.; J. Immunol. 165, 628 (2000)
  6. A new mechanism for IL-5-dependent helminth control: neutrophil accumulation and neutrophil-mediated worm encapsulation in murine filariasis are abolished in the absence of IL-5: K.M. Al-Qaoud, et al.; Int. Immunol. 12, 899 (2000)
  7. Rapid recruitment of inflammatory monocytes is independent of neutrophil migration: R.B. Henderson, et al.; Blood 102, 328 (2003)
  8. Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils: B. de Vries, et al.; J. Immunol. 170, 3883 (2003)
  9. Absence of cross-reactivity between murine Ly-6C and Ly-6G: S. Nagendra, et al.; Cytom. A 58, 195 (2004)
  10. Activation of Toll-Like Receptor (TLR)2, TLR4, and TLR9 in the Mammalian Cornea Induces MyD88-Dependent Corneal Inflammation: A.C. Johnson, et al.;  Invest. Ophthalmol. Vis. Sci. 46, 589 (2005)
  11. Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani: E. McFarlane, et al.; Infect. Immun. 76, 532 (2008)
  12. Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections: A. Navarini, et al.; PNAS 106, 7107 (2009)
  13. Analysis of innate defences against Plasmodium falciparum in immunodeficient mice:  A Ludovic, et al.; Malaria J. 9, 197 (2010)
  14. Role of Neutrophils in the Early Shaping of the Leishmania Major Specific Immune Response in Experimental Murine Cutaneous Leishmaniasis: M. Charmoy, et al.; Neutrophils in Infectious Diseases 1, 49 (2011)
  15. The Influence of Reactive Oxygen Species and Glucocorticoids on Dry Skin in a Mouse Model of Arthritis: K. Goto, et al.; Skin Pharmacol. Physiol. 31, 188 (2018)
  16. Intraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity: M. Yoshida, et al.; Int. J. Oncol., ahead of print (2018)




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