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品牌：AdipoGen       货号：AG-20B-0020-C100

      聚谷氨酰化是翻译后修饰，其中可变长度的谷氨酸侧链形成在修饰的蛋白质上。它在进化上是保守的，最突出的底物是微管蛋白，即微管（MT）构建基块。已经提出聚谷氨酰化参与MT的功能适应，因为它发生在羧基末端的微管蛋白尾巴中，其直接参与许多与MT相关的结构蛋白和运动蛋白的结合。最近对新的聚谷氨酰化底物的鉴定表明，这种翻译后修饰可能是多种细胞过程的潜在调节剂，并参与细胞周期和细胞增殖。

名称：聚麸胺修饰单抗 AG-20B-0020-C100
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品牌货号产品名称规格
AdopigenAG-20B-0020-C100

anti-Polyglutamylation Modification, mAb (GT335)

聚麸胺修饰单抗 （GT335）

100 µg

产品说明书： 

AG-20B-0020-anti-Polyglutamylation-Modification-mAb-GT335.pdf


Product Details
SynonymsPolyglutamylated Tubulin; Glutamylated Tubulin; Postranslational Protein Glutamylation
Product TypeMonoclonal Antibody


Properties

CloneGT335
IsotypeMouse IgG1κ
Source/HostPurified from concentrated hybridoma tissue culture supernatant.
Immunogen/AntigenOctapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk).
Application

Electron Microscopy (see Lit. 3)
Immunocytochemistry: (1:2000)
Immunohistochemistry: (paraffin sections; 1:1000)
Immunoprecipitation
Western Blot: (1:4000)
Optimal conditions must be determined individually for each application.

CrossreactivityAll
Specificity

Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated α- and β-tubulin.

Purity≥95% (SDS-PAGE)
Purity DetailProtein G-affinity purified.
Concentration1mg/ml
FormulationLiquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control

Mouse IgG1 Isotype Control

Other Product Data

Recognizes most forms of polyglutamylated tubulin (α- and β-tubulin), independent of the length of the glutamate side chains. No specificity to particular tubulin isoforms nor to tubulin from particular species are observed. Detects also other (poly)glutamylated proteins. Since no consensus modification site is known for protein (poly)glutamylation, the detection is not sequence-specific. However, an acidic environment of the modification site is required.
The use of the antibody at too high concentrations obscures its specificity in immunofluorescence.


Shipping and Handling

ShippingBLUE ICE
Short Term Storage+4°C
Long Term Storage-20°C
Handling AdviceAfter opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/StabilityStable for at least 1 year after receipt when stored at -20°C.


·参考文献

  1. Distribution of glutamylated alpha and beta-tubulin in mouse tissues using a specific monoclonal antibody, GT335: A. Wolff, et al.; Eur. J. Cell Biol. 59, 425 (1992)
  2. Polyglutamylation of nucleosome assembly proteins: C. Regnard, et al.; J. Biol. Chem. 275, 15969 (2000)
  3. Glutamylated tubulin: diversity of expression and distribution of isoforms: M.L. Kann, et al.; Cell Motil. Cytoskeleton 55, 14 (2003)
  4. Polyglutamylation Is a Post-translational Modification with a Broad Range of Substrates: J. van Dijk, et al.; J. Biol. Chem. 283, 3915 (2008)
  5. Unique post-translational modifications in specialized microtubule architecture: K. Ikegami & M. Setou; Cell Struct. Funct. 35, 15 (2010) (Review)
  6. Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells: S. Zink|et al.; J. Cell Sci. 125, 5998 (2012)
  7. The CP110-interacting proteins Talpid3 and Cep290 play overlapping and distinct roles in cilia assembly: T. Kobayashi, et al.; J. Cell Biol. 204, 215 (2014)
  8. CNS axons globally increase axonal transport after peripheral conditioning: F.M. Mar, et al.; J. Neurosci. 34, 5965 (2014)
  9. Microtubule detyrosination guides chromosomes during mitosis: M. Barisic, et al.; Science 348, 6236 (2015) (Supplement)
  10. SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture: M. Hilbert, et al.; Nat. Cell Biol. 18, 393 (2016)
  11. Loss of RPGR glutamylation underlies the pathogenic mechanism of retinal dystrophy caused by TTLL5 mutations: X. Sun, et al.; PNAS 113, E2925 (2016)
  12. Alterations in the balance of tubulin glycylation and glutamylation inphotoreceptors leads to retinal degeneration: M. Bosch Grau, et al.; J. Cell. Sci. 130, 938 (2017)
  13. The actin-MRTF-SRF transcriptional circuit controls tubulin acetylation via α-TAT1 gene expression: J. Fernández-Barrera, et al.; J. Cell Biol. 217, 929 (2018)
  14. iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications: J. Ou, et al.; Cell 173, 1 (2018)
  15. Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice: B. Ye, et al.; Nat. Commun. 9, 1261 (2018)
  16. Centrosome amplification arises before neoplasia and increases upon p53 loss in tumorigenesis: C.A.M. Lopes, et al.; J. Cell. Biol. 217, 2353 (2018)








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