产品说明书：

AG_20B_0014_Western_Blot_Protocol.pdf

AG-20B-0014-anti-NLRP3-NALP3-mAb-Cryo-2.pdf

·产品详情

别名：NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin; Cold Autoinflammatory Syndrome 1 Protein

产品类型：单抗

·产品属性

克隆号：Cryo-2

亚型：小鼠IgG2b

来源/宿主：从浓宿杂交瘤组织培养上清液中提取

应用：WB:Western印迹：（1ug/mL）

    ICC:1.see J.Virol.85，13019（2011）；2.Nat.Immunol.13，255（2012）

    IP：in RIPA buffer，see Mol.Cell 49，331（2012）

    IHC:1：200，for a protocol see Nature 493，67（2012）ChIP assay：2ug/DNA of 10E6 cells（see Nat.Immunol.16，859（2015））

反应性：人/小鼠

特异性：识别人和小鼠NLRP3/NALP3

纯度 ：≥95%（SDS-PAGE）

纯化细节：G蛋白亲和纯化

浓度：1mg/mL

配制：液体，溶解在含10%甘油和0.02%叠氮化钠的PBS缓中液中。

·运输及处理

运输条件：冰袭

短期储存：+4℃

长期储存：-20℃

处理建议：打开后，等分处理并储存于-20℃条件下。避免反复冻融。

使用/稳定性：收货后保存于-20℃条件下，可至少保存1年。

·参考文献

1. Thioredoxin-interacting protein links oxidative stress to inflammasome activation: R. Zhou, et al.; Nature Immunology 11, 136 (2010)
2. The NLRP3 inflammasome promotes renal inflammation and contributes to CKD: A. Vilaysane, et al.; J. Am. Soc. Nephrol. 21, 1732 (2010)
3. A role for mitochondria in NLRP3 inflammasome activation: R. Zhou, et al.; Nature 469, 221 (2011)
4. Differential expression of NLRP3 among hematopoietic cells: G. Guarda, et al.; J. Immunol. 186, 2529 (2011)
5. Mechanism of Impaired NLRP3 Inflammasome Priming by Monophosphoryl Lipid A: C.A. Embry, et al.; Sci. Signal. 4, ra28 (2011)
6. Measles Virus V Protein Inhibits NLRP3 Inflammasome-Mediated Interleukin-1 Secretion: N. Komune, et al.; J. Virol. 85, 13019 (2011)
7. Novel role of PKR in inflammasome activation and HMGB1 release: B. Lu, et al.; Nature 488, 670 (2012)
8. Deubiquitination of NLRP3 by BRCC3 Critically Regulates Inflammasome Activity: B.F. Py, et al.; Mol. Cell 49, 331 (2012)
9. NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.; Nature 493, 67 (2012)
10. Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinated inflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255 (2012)
11. Inflammasome-independent NLRP3 augments TGF-β signaling in kidney epithelium: W. Wang, et al.; J. Immunol. 190, 1239 (2013)
12. Omega-3 Fatty Acids Prevent Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome Activation: Y. Yan, et al.; Immunity 38, 1154 (2013)
13. LRRFIP2 negatively regulates NLRP3 inflammasome activation in macrophages by promoting Flightless-I-mediated caspase-1 inhibition: J. Jin, et al.; Nat. Commun. 4, 2075 (2013)
14. Proapoptotic Chemotherapeutic Drugs Induce Noncanonical Processing and Release of IL-1β via Caspase-8 in Dendritic Cells: C. Antonopoulus, et al. J. Immunol. 191, 4789 (2013)
15. Constitutively activated NLRP3 inflammasome causes inflammation and abnormal skeletal development in mice: S.L. Bonar, et al.; PLoS One 7, e35979 (2012)
16. FADD and caspase-8 mediate priming and activation of the canonical and noncanonical Nlrp3 inflammasomes: P. Gurung, et al.; J. Immunol. 192, 1835 (2014)
17. XIAP Restricts TNF- and RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell Rep. 7, 1796 (2014)
18. The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation: B.S. Franklin, et al.; Nat. Immunol. 15, 727 (2014)
19. The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014)
20. Toward a chemical marker for inflammatory disease: a fluorescent probe for membrane-localized thioredoxin: M.H. Lee, et al.; JACS 136, 8430 (2014)
21. Prostaglandin E2 inhibits NLRP3 inflammasome activation through EP4 receptor and intracellular cyclic AMP in human macrophages: M. Sokolowska, et al.; J. Immunol. 194, 5472 (2015)
22. The receptor NLRP3 is a transcriptional regulator of TH2 differentiation: M. Bruchard, et al.; Nat. Immunol. 16, 859 (2015)
23. NLRP3 Inflammasome Is Expressed and Functional in Mouse Brain Microglia but Not in Astrocytes: A. Gustin, et al.; PLos One 10, e0130624 (2015)
24. Nlrp6 promotes recovery after peripheral nerve injury independently of inflammasomes: E. Ydens, et al.; J. Neuroinfl. 12, 143 (2015)
25. Human monocytes engage an alternative inflammasome pathway: M.M. Gaidt, et al.; Immunity 44, 833 (2016)
26. Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking: S. Khare, et al.; Methods Mol. Biol. 1417, 131 (2016)
27. Functional Reconstruction of NLRs in HEK293 Cells: V. Compan & G. Lopez-Castejon; Methods Mol. Biol. 1417, 217 (2016)
28. The NLRP3 inflammasome and bruton's tyrosine kinase in platelets co-regulate platelet activation, aggregation, and in vitro thrombus formation: P. Murthy, et al.; BBRC 483, 230 (2017)
29. Pioglitazone Confers Neuroprotection Against Ischemia-Induced Pyroptosis due to its Inhibitory Effects on HMGB-1/RAGE and Rac1/ROS Pathway by Activating PPAR-γ: P. Xia, et al.; Cell Physiol. Biochem. 45, 2351 (2018)
30. The Crohn’s disease risk factor IRGM limits NLRP3 inflammasome activation by impeding its assembly and by mediating its selective autophagy: S. Mehto, et al.; Mol. Cell 73, 429 (2019)
31. Lack of Protein Prenylation Promotes Nlrp3 Inflammasome Assembly in Human Monocytes: O.P. Skinner, et al.; J. Allergy Clin. Immunol. 143, 2315(2019) [KO validation]
32. Cathepsin B Is Required for NLRP3 Inflammasome Activation in Macrophages, Through NLRP3 Interaction: A. Chevriaux, et al.; Front. Cell. Dev. Biol. (Epub ahead of print) (2020)
33. Caspase-6 Is a Key Regulator of Innate Immunity, Inflammasome Activation, and Host Defense: M. Zheng, et al.; Cell (Epub ahead of print) (2020)