AG-20B-0010-anti-ZBP1-mAb-Zippy-1.pdf

·产品详情

别名：Z-DNA结合蛋白1；肿瘤基质和活化巨噬细胞蛋白DLM-1；DNA依赖性干扰素调节因子激活剂；DAl

产品类型：单抗

·产品属性

克隆号：Zippy-1

亚型：小鼠IgG2a

来源/宿主：从浓宿杂交瘤组织培养上清液中提取

免疫原/抗原|重组小鼠ZBP1（aa 1-411）

应用：IHC:（1：500）

    IP:（1：200）

    WB:（1：1，000）

反应性：人/小鼠

特异性：识别人和小鼠ZBP1

纯度 ：≥95%（SDS-PAGE）

纯化细节：G蛋白亲和纯化

浓度：1mg/mL

配制：液体，溶解在含10%甘油和0.02%叠氮化钠的PBS缓中液中。

·运输及处理

运输条件：冰袭

短期储存：+4℃

长期储存：-20℃

处理建议：打开后，等分处理并储存于-20℃条件下。避免反复冻融。

使用/稳定性：收货后保存于-20℃条件下，可至少保存1年。

·参考文献

1. ZBP1/DAI is an innate sensor of influenza virus triggering the NLRP3 inflammasome and programmed cell death pathways: T. Kuriakose, et al.; Sci. Immunol. 1, aag2045 (2016)
2. DAI senses Influenza A virus genomic RNA and activates RIPK3-dependent cell death: R.J. Thapa, et al.; Cell Host & Microbe 20, 674 (2016)
3. RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation: J. Lin, et al.; Nature 540, 124 (2016)
4. RIPK1 inhibits ZBP1-driven necroptosis during development: K. Newton, et al.; Nature 540, 129 (2016)
5. IRF1 Is a Transcriptional Regulator of ZBP1 Promoting NLRP3 Inflammasome Activation and Cell Death during Influenza Virus Infection: T. Kuriakose, et al.; J. Immunol. 200, 1489 (2018)
6. Species-independent contribution of ZBP1/DAI/DLM-1-triggered necroptosis in host defense against HSV1: H. Guo, et al.; Cell Death Dis. 9, 816 (2018) [KO Validation]
7. Constitutive interferon signaling maintains critical threshold of MLKL expression to license necroptosis: J. Sarhan, et al.; Cell Death Diff. 26, 332 (2019)
8. ZBP1/DAI Drives RIPK3-Mediated Cell Death Induced by IFNs in the Absence of RIPK1: J.P. Ingram, et al.; J. Immunol. 203, 1348 (2019)
9. IRF3 and IRF7 contribute to diesel exhaust particles-induced pulmonary inflammation by mediating mTORC1 activation and restraining autophagy in mice: C. Wang, et al.; Eur. J. Immunol. (Epub ahead of print) (2020)
10. Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis: T. Zhang, et al.; Cell 180, 1115 (2020)
11. Z-nucleic-acid sensing triggers ZBP1-dependent necroptosis and inflammation: H. Jiao, et al.; Nature 580, 391 (2020)
12. Caspase-6 Is a Key Regulator of Innate Immunity, Inflammasome Activation, and Host Defense: M. Zheng, et al.; Cell (Epub ahead of print) (2020)