说明书:

TRAP/ALP染色试制盒说明书

TRAP/ALP染色试剂盒

骨组织染色

       正常的骨代谢是通过成骨细胞生长与破骨细胞建立骨吸收而维持平衡。成骨细胞的标记酶是碱性磷酸酶（ALP）。破骨细胞的标记酶是抗酒石酸酸性磷酸酶（TRAP）。这两种标记酶在组织切片或者培养细胞过程中，被作为成骨细胞、破骨细胞存在的标记指标。

    本试剂盒可通过骨组织切片以及培养细胞中TRAP/ALP酶活性进行组织染色。通过观察成骨细胞和破骨细胞的染色成像，可检查细胞的分化状态和骨组织的分布情况。

·特点

·使用时混合3种溶液，可制备TRAP酶活性染色的显色底物溶液。

·ALP酶活性染色使用的是预混物溶液，操作简便。

·TRAP的活性部位呈红紫色，ALP的活性部位呈蓝色（茶褐色），可双重染色。

·适用于骨组织切片（脱钙GMA树脂包埋切片）以及培养细胞。

*sigma的387A（TRAP染色试剂盒）有货，详情请点击

·试剂盒组成

·酒石酸溶液（×10）……3mL

·酸性磷酸酶底物液A……30mL

·酸性磷酸酶底物液B.……3mL

·核染色试剂…10mL

·碱性磷酸酶预混物溶液···30mL

<备注>本产品可对应培养细胞包装24孔板-5次，96孔板-6次。

骨组织载玻片（一个载玻片约使用500uL）包装为60个。

·培养细胞TRAP/ALP酶活性染色案例

·用TRAP染色剂使破骨细胞呈红色

·用ALP染色剂使成骨细胞的细胞膜、软骨细胞和细胞间膜呈茶褐色

·用核染色试剂使各种细胞核呈蓝绿色。

    RAW264细胞的TRAP活性染色                        MC3T3-E1细胞ALP活性染色

相关资料：石蜡切片骨相关酶（TRAP，ALP）双重染色步骤

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